Effect of Rat Placental Culture Supernatants on Cellular and Humoral Immune Responses
| Autor: | Ileana Malan Borel, Songeli Menezes Freire, Ricardo A. Margni, Andrea Canellada |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Cytotoxicity
Immunologic Male Cellular immunity medicine.medical_specialty Proliferation index Placenta Immunology chemical and pharmacologic phenomena Lymphocyte Activation Mice Pregnancy Culture Techniques Internal medicine Splenocyte medicine Animals Immunology and Allergy Rats Wistar Cytotoxicity Immunity Cellular Mice Inbred BALB C Hybridomas biology Cell growth Obstetrics and Gynecology Molecular biology Rats Inbred F344 In vitro Rats Endocrinology Reproductive Medicine Antibody Formation Humoral immunity biology.protein Female Lymphocyte Culture Test Mixed Antibody Spleen |
| Zdroj: | American Journal of Reproductive Immunology. 38:366-373 |
| ISSN: | 1046-7408 |
| Popis: | PROBLEM: To evaluate the effect of rat placental culture supernatants (PS) on spontaneous, mitogen- and alloantigen-induced lymphoproliferation, antibody synthesis regulation, and symmetric/asymmetric antibody ratio. METHOD Of STUDY: The effect of PS was determined: (a) on cell proliferation of murine hybridoma cells and on spontaneous or ConA-induced proliferation of murine and rat splenocytes by thymidine incorporation; (b) on rat or mouse cell-mediated cytotoxicity (CMC) by 51Cr release; and (c) on antibody synthesis by enzyme-linked immunoadsorbent assay (ELISA). RESULTS: With 20% PS, hybridoma cell inhibition was 37% and that of splenocytes up to 60%, whereas it was 75 and 43%, respectively, in the presence of ConA. Despite marked cell death, hybridoma proliferation index increased significantly. There was a drop in total anti-dinitrophenylated (DNP) immunoglobulin Gl (IgG1) antibody production and an increase in asymmetric antibody percentage, correlating with placental supernatant concentration. CONCLUSIONS: Rat placental culture supernatants inhibit cell proliferation in all cases, diminish total antibody production, and increase the percentage of asymmetric antibodies by the hybridoma, and they increase antibody production by rat splenocytes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text