Substrate geometry directs the in vitro mineralization of calcium phosphate ceramics

Autor: Sander C.G. Leeuwenburgh, Pamela Habibovic, Zeinab Tahmasebi Birgani, Jeroen J.J.P. van den Beucken, Anna Tampieri, Maurilio Marcacci, Joop G.C. Wolke, Michele Bianchi, John A. Jansen, Eva R. Urquia Edreira
Přispěvatelé: Division Instructive Biomaterials Eng, RS: MERLN - Instructive Biomaterials Engineering (IBE), Faculty of Science and Technology
Rok vydání: 2014
Předmět:
beta-Tricalcium phosphate
Calcium Phosphates
Ceramics
X-Ray Emission
Materials science
Mineralization
Scanning electron microscope
Simulated body fluid
Biomedical Engineering
Nucleation
Energy-dispersive X-ray spectroscopy
chemistry.chemical_element
Mineralogy
Other Research Radboud Institute for Molecular Life Sciences [Radboudumc 0]
Calcium
Biochemistry
Mineralization (biology)
Electron
Calcium phosphates
Concavities
Hydroxyapatite
β-Tricalcium phosphate
Body Fluids
Microscopy
Electron
Scanning
Minerals
Spectrometry
X-Ray Emission
Temperature
X-Ray Diffraction
Biomaterials
chemistry.chemical_compound
Scanning
beta-Tricalcium phosphate
Concavities
Molecular Biology
Microscopy
METIS-298495
Spectrometry
Substrate (chemistry)
General Medicine
Phosphate
Reconstructive and regenerative medicine Radboud Institute for Molecular Life Sciences [Radboudumc 10]
chemistry
Chemical engineering
IR-87637
Biotechnology
Zdroj: Acta Biomaterialia, 10, 661-9
Acta Biomaterialia, 10, 2, pp. 661-9
Acta Biomaterialia, 10(2), 661-669. Elsevier / Bunge
Acta biomaterialia, 10(2), 661-669. Elsevier
Acta biomaterialia 10 (2014): 661–669. doi:10.1016/j.actbio.2013.10.026
info:cnr-pdr/source/autori:Bianchi, Michele; Edreira, Eva R. Urquia; Wolke, Joop G. C.; Birgani, Zeinab T.; Habibovic, Pamela; Jansen, John A.; Tampieri, Anna; Marcacci, Maurilio; Leeuwenburgh, Sander C. G.; van den Beucken, Jeroen J. J. P./titolo:Substrate geometry directs the in vitro mineralization of calcium phosphate ceramics/doi:10.1016%2Fj.actbio.2013.10.026/rivista:Acta biomaterialia/anno:2014/pagina_da:661/pagina_a:669/intervallo_pagine:661–669/volume:10
ISSN: 1742-7061
DOI: 10.1016/j.actbio.2013.10.026
Popis: Repetitive concavities on the surface of bone implants have recently been demonstrated to foster bone formation when implanted at ectopic locations in vivo. The current study aimed to evaluate the effect of surface concavities on the surface mineralization of hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) ceramics in vitro. Hemispherical concavities with different diameters were prepared at the surface of HA and beta-TCP sintered disks: 1.8 mm (large concavity), 0.8 mm (medium concavity) and 0.4 mm (small concavity). HA and beta-TCP disks were sintered at 1100 or 1200 degrees C and soaked in simulated body fluid for 28 days at 37 degrees C; the mineralization process was followed by scanning electron microscopy, energy-dispersive spectroscopy, X-ray diffraction and calcium quantification analyses. The results showed that massive mineralization occurred exclusively at the surface of HA disks treated at 1200 degrees C and that nucleation of large aggregates of calcium phosphate started specifically inside small concavities instead of on the planar surface of the disks. Regarding the effect of concavity diameter size on surface mineralization, it was observed that small concavities induce 124- and 10-fold increased mineralization compared to concavities of large or medium size, respectively. The results of this study demonstrated that (i) in vitro surface mineralization of calcium phosphate ceramics with surface concavities starts preferentially within the concavities and not on the planar surface, and (ii) concavity size is an effective parameter to control the spatial position and extent of mineralization in vitro. (C) 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
Databáze: OpenAIRE
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