Microvesicles released from human embryonic stem cell derived-mesenchymal stem cells inhibit proliferation of leukemia cells
| Autor: | Xiang Chen, Xiang-Mei Wen, Lei Zhang, Yongbin Ma, Kai Ye, Jianyi Gao, Yao Dai, Xianyan Ji, Fuhao Qiao, Hui Wang, Jiabo Hu, Yuan Ji, Jiang Lin |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cellular differentiation Human Embryonic Stem Cells Apoptosis Biology 03 medical and health sciences Cell-Derived Microparticles Cancer stem cell Autophagy Humans Cells Cultured Cell Proliferation Leukemia Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Embryonic stem cell Microvesicles Cell biology Endothelial stem cell 030104 developmental biology Oncology embryonic structures biological phenomena cell phenomena and immunity Stem cell Adult stem cell |
| Zdroj: | Oncology Reports. 38:1013-1020 |
| ISSN: | 1791-2431 1021-335X |
| Popis: | Human embryonic stem cell derived-mesenchymal stem cells (hESC‑MSCs) are able to inhibit proliferation of leukemia cells. Microvesicles released from human embryonic stem cell derived-mesenchymal stem cells (hESC‑MSC‑MVs) might play an important part in antitumor activity. Microvesicles were isolated by ultracentrifugation and identified under a scanning electron microscopy and transmission electron microscope separately. After 48-h cocultured with hESC‑MSCs and hESC‑MSC‑MVs, the number of K562 and HL60 was counted and tumor cell viability was measured by CCK8 assay. The expression of proteins Bcl-2 and Bax were estimated by western blotting. Transmission electron microscope and western blot analysis were adopted to evaluate the autophagy level. Results showed that both hESC‑MSCs and hESC‑MSC‑MVs inhibited proliferation of leukemia cells in a concentration-dependent manner. hESC‑MSC‑MVs reduced the ratio of Bcl/Bax, enhanced the protein level of Beclin-1 and LC3-II conversion, thus upregulating autophagy and apoptosis. In conclusion, microvesicles released from human embryonic stem cell derived-mesenchymal stem cells inhibited tumor growth and stimulated autophagy and excessive autophagy might induce apoptosis. |
| Databáze: | OpenAIRE |
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