Common Virulence Factors and Genetic Relationships between O18:K1:H7 Escherichia coli Isolates of Human and Avian Origin
Autor: | Annie Brée, Miguel Blanco, Pierre Germon, Jacques Mainil, Eric Oswald, Maryvonne Moulin-Schouleur, Jorge Blanco, Mu-Rong Kao, Catherine Schouler, Patrick Tailliez |
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Přispěvatelé: | Infectiologie Animale et Santé Publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Ecologie microbienne des insectes et interactions hôte-pathogène (EMIP), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université de Liège, Universidade de Santiago de Compostela [Spain] (USC ) |
Rok vydání: | 2006 |
Předmět: |
Microbiology (medical)
Serotype animal structures Virulence Factors Fimbria Virulence Biology medicine.disease_cause Microbiology 03 medical and health sciences chemistry.chemical_compound Genotype Escherichia coli Pulsed-field gel electrophoresis medicine Animals Humans Phylogeny 030304 developmental biology 0303 health sciences 030306 microbiology Bacteriology Bacterial adhesin [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology chemistry Aerobactin Chickens |
Zdroj: | Journal of Clinical Microbiology Journal of Clinical Microbiology, American Society for Microbiology, 2006, 44 (10), pp.3484-3492. ⟨10.1128/JCM.00548-06⟩ |
ISSN: | 1098-660X 0095-1137 |
Popis: | Extraintestinal pathogenic (ExPEC) Escherichia coli strains of serotype O18:K1:H7 are mainly responsible for neonatal meningitis and sepsis in humans and belong to a limited number of closely related clones. The same serotype is also frequently isolated from the extraintestinal lesions of colibacillosis in poultry, but it is not well known to what extent human and avian strains of this particular serotype are related. Twenty-two ExPEC isolates of human origin and 33 isolates of avian origin were compared on the basis of their virulence determinants, lethality for chicks, pulsed-field gel electrophoresis (PFGE) patterns, and classification in the main phylogenetic groups. Both avian and human isolates were lethal for chicks and harbored similar virulence genotypes. A major virulence pattern, identified in 75% of the isolates, was characterized by the presence of F1 variant fimbriae; S fimbriae; IbeA; the aerobactin system; and genomic fragments A9, A12, D1, D7, D10, and D11 and by the absence of P fimbriae, F1C fimbriae, Afa adhesin, and CNF1. All but one of the avian and human isolates also belonged to major phylogenetic group B2. However, various subclonal populations could be distinguished by PFGE in relation to animal species and geographical origin. These results demonstrate that very closely related clones can be recovered from extraintestinal infections in humans and chickens and suggest that avian pathogenic E. coli isolates of serotype O18:K1:H7 are potential human pathogens. |
Databáze: | OpenAIRE |
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