miR-125a-5p suppresses colorectal cancer progression by targeting VEGFA
| Autor: | Xuehua Yang, Junbo Qian, Yaming Wang, Haifeng Kang, Jianwei Qiu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
VEGFA
0301 basic medicine Tube formation Cell growth Colorectal cancer overall survival colorectal cancer miR-125a-5p Biology medicine.disease In vitro Metastasis 03 medical and health sciences Vascular endothelial growth factor A 030104 developmental biology 0302 clinical medicine Oncology In vivo Cancer Management and Research 030220 oncology & carcinogenesis Cancer research medicine Human umbilical vein endothelial cell Original Research |
| Zdroj: | Cancer Management and Research |
| ISSN: | 1179-1322 |
| Popis: | Xuehua Yang, Jianwei Qiu, Haifeng Kang, Yaming Wang, Junbo Qian Department of Gastroenterology, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu, China Background: MiR-125a-5p has been reported to be involved in the development and progression of various cancers. However, the biological function and underlying mechanisms in colorectal cancer(CRC) still remain unclear. Here, we explored the potential biological roles of miR-125a-5p in CRC. Methods: The expression of miR-125a-5p was detected using quantitative real-time PCR (qRT-PCR), biological functions of miR-125a-5p were assessed by cell counting kit-8, wound-healing, transwell invasion, and human umbilical vein endothelial cell (HUVEC) tube formation assays in vitro and animal experiments in vivo. Results: We found that miR-125a-5p was downregulated in CRC tissues and cell lines, it inhibited CRC cell proliferation, migration, and invasion and reduced the ability of HUVECs to form tubes. Moreover, we verifed that miR-125a-5p suppressed CRC growth and metastasis in vivo. Additionally, we showed that VEGFA, a direct target gene of miR-125a-5p, could reverse the inhibitory effect caused by miR-125a-5p overexpression.Conclusion: miR-125a-5p might serve as a tumor suppressor in CRC and could be regarded as a potential therapeutic candidate for CRC. Keywords: miR-125a-5p, colorectal cancer, VEGFA, overall survival |
| Databáze: | OpenAIRE |
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