Androgen biosynthesis in the stomach: expression of cytochrome P450 17 alpha-hydroxylase/17,20-lyase messenger ribonucleic acid and protein, and metabolism of pregnenolone and progesterone by parietal cells of the rat gastric mucosa
| ISSN: | 0013-7227 |
|---|---|
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce3a0d6ba37ac425ef57214bd6ff83c2 https://pubmed.ncbi.nlm.nih.gov/7895686 |
| Přírůstkové číslo: | edsair.doi.dedup.....ce3a0d6ba37ac425ef57214bd6ff83c2 |
| Autor: | M. Gouézou, Etienne-Emile Baulieu, N. Sananès, C. Le Goascogne, Bernard Eychenne, Paul Robel |
| Rok vydání: | 1995 |
| Předmět: |
Male
medicine.medical_specialty Molecular Sequence Data Androstenediol Dehydroepiandrosterone Gene Expression Biology Rats Sprague-Dawley chemistry.chemical_compound Endocrinology Cytochrome P-450 Enzyme System Parietal Cells Gastric Internal medicine medicine Gastric mucosa Animals Testosterone Cholesterol Side-Chain Cleavage Enzyme RNA Messenger Progesterone Androgen biosynthetic process Base Sequence Cholesterol side-chain cleavage enzyme Androstenedione Steroid 17-alpha-Hydroxylase Rats medicine.anatomical_structure chemistry Gastric Mucosa Pregnenolone Androgens Gastric acid Female medicine.drug |
| Zdroj: | Endocrinology. 136(4) |
| ISSN: | 0013-7227 |
| Popis: | Dehydroepiandrosterone (DHEA) and its conjugates persist in the rat brain, for up to 1 month after ablation of both adrenals and gonads. Since DHEA synthesis in brain from pregnenolone (PREG) was excluded, we have considered other tissular sources including the digestive tract. In situ hybridization with specific oligonucleotide probes showed that the parietal cells of the gastric mucosa, contrary to other cell types, strongly expressed P450(17) alpha messenger RNA. Expression of the enzyme in the parietal cells was confirmed by immunocytochemistry with specific antibodies. An intense reaction was observed in the stomach of adult males and of cyclic or pregnant females. Access to food did not influence the intensity of immunostaining. It appeared at postnatal days 16-21, then the number of positive cells increased rapidly and leveled off at adult age. Parietal cells were released by pronase digestion of everted stomachs from adult male and female rats and were purified by density gradient centrifugation on Nycodenz. 5 x 10(4) to 1.6 x 10(6) cells were incubated with either 1 microM 14C-PREG or 14C-progesterone (14C-PROG) at 37 C under 95% O2-5% CO2, for 10-180 min. PREG was converted to 17-OH PREG and to androstenediol, whereas PROG was converted to 17-OH PROG and to testosterone. Only minute amounts of either DHEA or androstenedione, respectively, were detected at any incubation time, indicating their fast conversion to the corresponding 17 beta-hydroxysteroids. 3H-25-OH cholesterol was not metabolized to 3H-PREG, and 14C-PREG was not converted to 14C-PROG, in accordance with negative immunocytochemical results with antibodies to cytochrome P450scc and 3 beta-hydroxysteroid dehydrogenase delta 5-->4-isomerase (3 beta-HSD). In conclusion, the parietal cells, which are known as the source of gastric acid secretion, can synthesize testosterone from PROG and androstenediol from PREG. The physiological relevance of such conversions remains to be established. |
| Databáze: | OpenAIRE |
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