Prevalence and phylogenetic analysis of HTLV-1 in blood donors in Golestan Province, in the Northeast of Iran
| Autor: | Zohreh Sharifi, Asma Bahrami, Ali Akbar Pourfathollah, Masoud Parsania, Hossein Mehrabi Habibabadi |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
viruses 030106 microbiology Blood Donors Iran Biology Virus 03 medical and health sciences Western blot immune system diseases Virology Genotype Prevalence medicine Humans Phylogeny Human T-lymphotropic virus 1 Phylogenetic tree medicine.diagnostic_test HTLV-I Infections genomic DNA 030104 developmental biology Virus type Elisa test Nested polymerase chain reaction |
| Zdroj: | Journal of Virological Methods. 290:114073 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2021.114073 |
| Popis: | The human T-lymphotropic virus type 1 (HTLV-1) can cause ATL or TSP. This study evaluates the prevalence of HTLV-1 infection in blood donors in Golestan province. The study was conducted among 4226 blood donors and ELISA test was performed for the initial HTLV-1 screening. Reactive samples were confirmed by Western blot and Electrochemiluminescence tests. Then recalling donors with reactive results was done and genomic DNA from the new sample was extracted and tested using the Nested PCR method and phylogenetic analysis was performed. At first, 8 samples were reactive with ELISA test and 4 samples were confirmed with western blot, Electrochemiluminescence and Nested PCR tests.The sequences of isolates was related to the HTLV-1 virus and subtype a (cosmopolitan) subgroup A.The prevalence of HTLV-1 virus in Golestan province was about 0.09 %.The genotype of virus isolates had a common ancestor with isolates of the Khorasan region. |
| Databáze: | OpenAIRE |
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