Erythropoietin Stimulates Normal Endothelial Progenitor Cell-Mediated Endothelial Turnover, but Attributes to Neovascularization Only in the Presence of Local Ischemia

Autor: Regien G. Schoemaker, B. Daan Westenbrink, Hisko Oeseburg, Adriaan A. Voors, Lennaert Kleijn, Wiek H. van Gilst, Rudolf A. de Boer, Dirk J. van Veldhuisen, Anne M. S. Belonje, Pim van der Harst
Přispěvatelé: Cardiovascular Centre (CVC), Schoemaker lab
Rok vydání: 2008
Předmět:
Male
DARBEPOETIN-ALPHA
Myocardial Infarction
heart failure
Ventricular Function
Left
Neovascularization
endothelial function
Cell Movement
DOXORUBICIN-INDUCED CARDIOMYOPATHY
Medicine
Darbepoetin alfa
Pharmacology (medical)
Bone Marrow Transplantation
IMPROVES CARDIAC-FUNCTION
Stem Cells
General Medicine
Coronary Vessels
CHRONIC HEART-FAILURE
Isoenzymes
Vasodilation
Endothelial stem cell
medicine.anatomical_structure
cardiovascular system
Cardiology
erythropoietin
Rats
Transgenic
medicine.symptom
Cardiology and Cardiovascular Medicine
medicine.drug
EXPRESSION
Cardiac function curve
medicine.medical_specialty
Endothelium
BONE-MARROW
Ischemia
Neovascularization
Physiologic
GPI-Linked Proteins
Endothelial progenitor cell
RATS
Internal medicine
Ventricular Pressure
Animals
Humans
NITRIC-OXIDE SYNTHASE
Progenitor cell
endothelial progenitor cells
Pharmacology
business.industry
Myocardium
Endothelial Cells
Alkaline Phosphatase
medicine.disease
Myocardial Contraction
Rats
Inbred F344
DYSFUNCTION
Capillaries
Disease Models
Animal
Endocrinology
MYOCARDIAL-INFARCTION
Erythropoietin
Angiogenesis Inducing Agents
business
Zdroj: Cardiovascular Drugs and Therapy, 22(4), 265-274. SPRINGER
ISSN: 1573-7241
0920-3206
DOI: 10.1007/s10557-008-6094-y
Popis: Purpose We aimed to evaluate whether ischemia is required for erythropoietin (EPO) induced stimulation of endothelial progenitor cells (EPCs) and their related effects on endothelial and cardiac function. Methods Bone marrow of rats was replaced by transgenic cells to allow tracking of EPCs. Ischemic heart failure was induced by left coronary artery ligation to induce myocardial infarction (MI) and control rats received a sham procedure. Three weeks after surgery, rats were randomized to receive EPO (darbepoetin alfa 40 μg/kg per 3 weeks) or vehicle and were sacrificed 9 weeks after surgery. Results In all treated groups, EPO significantly increased circulating EPCs and their incorporation into the endothelium of the ischemic and non-ischemic hearts as well as in the control organs; kidney and liver. This was associated with significantly improved endothelial function, which was strongly correlated with circulating EPCs (R=0.7, p< 0.01). However, additional EPCs preferentially homed to the ischemic MI borderzone (p
Databáze: OpenAIRE
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