Toxin-neutralizing antibodies elicited by naturally acquired cutaneous anthrax are elevated following severe disease and appear to target conformational epitopes

Autor: Lori Garman, Hayati Demiraslan, Judith A. James, A. Darise Farris, Geert-Jan Boons, Rebecca J. Ingram, Uner Kayabas, Eric K. Dumas, Jimmy D. Ballard, Jason L. Larabee, Emily E. Muns, Rebecca M Sparks, Mehmet Doganay, Adriana Zamora
Přispěvatelé: Afd Chemical Biology and Drug Discovery, Sub Chemical Biology and Drug Discovery, Chemical Biology and Drug Discovery
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Bacterial Diseases
Male
0301 basic medicine
Turkey
Physiology
Humoral Immune Response
Bacillus
Anthrax Vaccines
Toxicology
Pathology and Laboratory Medicine
medicine.disease_cause
Biochemistry
Epitope
Epitopes
0302 clinical medicine
Antigen Encapsulation
Antibody Specificity
Zoonoses
Immune Physiology
Microbial Physiology
Medicine and Health Sciences
Toxins
Edema
Bacterial Physiology
030212 general & internal medicine
Drug Delivery System Preparation
Immune System Proteins
Multidisciplinary
Pharmaceutics
Middle Aged
Antibodies
Bacterial
Bacterial Pathogens
Bacillus anthracis
Infectious Diseases
Medical Microbiology
Medicine
Female
Pathogens
Antibody
Research Article
Adult
Science
Anthrax toxin
Toxic Agents
Immunology
Bacterial Toxins
Bacillus Anthracis
Biology
Microbiology
Antibodies
Anthrax
Young Adult
03 medical and health sciences
Signs and Symptoms
Immune system
Antigen
Diagnostic Medicine
Neutralization Tests
Immunity
medicine
Humans
Bacterial Spores
Microbial Pathogens
Antigens
Bacterial
Bacteria
Pharmaceutical Processing Technology
Toxin
fungi
Organisms
Biology and Life Sciences
Proteins
Bacteriology
Skin Diseases
Bacterial
biology.organism_classification
Antibodies
Neutralizing
Immunity
Humoral
030104 developmental biology
Immunoglobulin G
Humoral Immunity
biology.protein
Zdroj: PLoS One, 15(4), 1. Public Library of Science
PLoS ONE
Dumas, E K, Demiraslan, H, Ingram, R J, Sparks, R M, Muns, E, Zamora, A, Larabee, J, Garman, L, Ballard, J D, Boons, G-J, James, J A, Kayabas, U, Doganay, M & Farris, A D 2020, ' Toxin-neutralizing antibodies elicited by naturally acquired cutaneous anthrax are elevated following severe disease and appear to target conformational epitopes ', PLoS ONE, vol. 15, no. 4, e0230782 . https://doi.org/10.1371/journal.pone.0230782
PLoS ONE, Vol 15, Iss 4, p e0230782 (2020)
ISSN: 1932-6203
Popis: Understanding immune responses to native antigens in response to natural infections can lead to improved approaches to vaccination. This study sought to characterize the humoral immune response to anthrax toxin components, capsule and spore antigens in individuals (n = 46) from the Kayseri and Malatya regions of Turkey who had recovered from mild or severe forms of cutaneous anthrax infection, compared to regional healthy controls (n = 20). IgG antibodies to each toxin component, the poly-γ-D-glutamic acid capsule, the Bacillus collagen-like protein of anthracis (BclA) spore antigen, and the spore carbohydrate anthrose, were detected in the cases, with anthrax toxin neutralization and responses to Protective Antigen (PA) and Lethal Factor (LF) being higher following severe forms of the disease. Significant correlative relationships among responses to PA, LF, Edema Factor (EF) and capsule were observed among the cases. Though some regional control sera exhibited binding to a subset of the tested antigens, these samples did not neutralize anthrax toxins and lacked correlative relationships among antigen binding specificities observed in the cases. Comparison of serum binding to overlapping decapeptides covering the entire length of PA, LF and EF proteins in 26 cases compared to 8 regional controls revealed that anthrax toxin-neutralizing antibody responses elicited following natural cutaneous anthrax infection are directed to conformational epitopes. These studies support the concept of vaccination approaches that preserve conformational epitopes. Copyright: © 2020 Dumas et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Databáze: OpenAIRE
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