Massive Parallelization of STED Nanoscopy Using Optical Lattices
| Autor: | Bin Yang, Jean-Baptiste Trebbia, Brahim Lounis, Frédéric Przybilla, Michael Mestre |
|---|---|
| Přispěvatelé: | Laboratoire Photonique, Numérique et Nanosciences (LP2N), Université Sciences et Technologies - Bordeaux 1-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), lp2n-01,lp2n-12, Université Sciences et Technologies - Bordeaux 1-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Physics
[PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics] [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph] Resolution (electron density) STED microscopy FOS: Physical sciences Parallel computing Frame rate Atomic and Molecular Physics and Optics Pixelation Interference (communication) Biological Physics (physics.bio-ph) Microscopy Stimulated emission Physics - Biological Physics Image resolution Optics (physics.optics) Physics - Optics |
| Zdroj: | Optics Express Optics Express, Optical Society of America-OSA Publishing, 2014, 22 (5), pp.5581-5589. ⟨10.1364//OE.22.005581⟩ |
| ISSN: | 1094-4087 |
| DOI: | 10.1364//OE.22.005581⟩ |
| Popis: | Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-‐scanning. This constitutes a drawback for wide field imaging, since the gain in spatial resolution requires dense pixelation and hence long recording times. Here we achieve massive parallelization of STED nanoscopy using wide-‐field excitation together with well-‐designed optical lattices for depletion and a fast camera for detection. Acquisition of large field of view super-‐resolved images requires scanning over a single unit cell of the optical lattice which can be as small as 290 nm*290nm. Interference STED (In-‐STED) images of 2.9 μm* 2.9 μm with resolution down to 70 nm are obtained at 12.5 frames per second. The development of this technique opens many prospects for fast wide-‐field nanoscopy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|