Anti-apoptotic and anti-inflammatory activity of Gentiana lutea root extract

Autor: Filippo Maggi, Mariagrazia Perilli, Argante Bozzi, Giovanni Caprioli, Veronica Carnicelli, Gianfranco Amicosante, Teresa Cafaro, Giuseppe Celenza, Fabrizia Brisdelli
Rok vydání: 2020
Předmět:
Zdroj: Advances in Traditional Medicine. 20:619-630
ISSN: 2662-4060
2662-4052
DOI: 10.1007/s13596-020-00447-5
Popis: Gentiana lutea roots have been widely used in the traditional medicine, especially for their stomachic properties. In this study, the effect of G. lutea root methanol extract on SH-SY5Y cell survival and on anti-proliferative activity of pro-apoptotic agents was evaluated. In neuroblastoma cell line SH-SY5Y, the extract did not exhibit any cytotoxic activity, but protected cells from vinblastine-induced apoptosis. In the combined treatment with 200 µg/ml extract plus 0.1 µM vinblastine, extract significantly increased cell survival, reduced the percentage of apoptotic cells and decreased caspase-3 activity if compared with the vinblastine alone treatment. The anti-apoptotic activity of the extract was associated to the down-regulation of Sirt-1 protein and to the increase of anti-apoptotic Bcl-2 protein expression and intracellular GSH levels. Since the apoptosis modulation was evidenced on a neuroblastoma cell line, G. lutea extract could have a promising potential as a neuroprotective agent. Moreover, G. lutea root extract exhibited anti-inflammatory activity, since it inhibited LPS-induced expression of TNF-α in macrophage RAW264.7 cells.
Databáze: OpenAIRE
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