Impact of surface modification with gold nanoparticles on the bioelectrocatalytic parameters of immobilized bilirubin oxidase
| Autor: | Dmitry Pankratov, Sergey Shleev, Galina Pankratova, Dmitry N. Zajtsev, Yulia M. Parunova, Yulia S. Zeifman, M. E. Khlupova, Nina F. Bashirova, Alexandra V. Dudareva, Vladimir Popov |
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| Předmět: |
bioelectrocatalysis
bilirubin oxidase Standard hydrogen electrode Chemistry Scanning electron microscope Nanoparticle Electrochemistry Biochemistry Absorbance Electron transfer Colloidal gold Naturvetenskap GOLD NANOPARTICLE BILIRUBIN OXIDASE DIRECT ELECTRON TRANSFER BIOELECTROCATALYSIS Molecular Medicine direct electron transfer Natural Sciences Bilirubin oxidase Molecular Biology gold nanoparticle Biotechnology Nuclear chemistry |
| Zdroj: | Scopus-Elsevier ResearcherID |
| Popis: | We unveil experimental evidence that put into question the widely held notion concerning the impact of nanoparticles on the bioelectrocatalytic parameters of enzymatic electrodes. Comparative studies of the bio- electrocatalytic properties of fungal bilirubin oxidase from Myrothecium verrucaria adsorbed on gold electrodes, modified with gold nanoparticles of different diameters, clearly indicate that neither the direct electron transfer rate (standard heterogeneous electron transfer rate constants were calculated to be 31±9 s -1 ) nor the biocatalytic activity of the adsorbed enzyme (bioelectrocatalytic constants were calculated to be 34±11 s -1 ) depends on the size of the nanoparticles, which had diameters close to or larger than those of the enzyme molecules. KeyWordS gold nanoparticle; bilirubin oxidase; direct electron transfer; bioelectrocatalysis. abbreViationS 3D - three-dimensional; A real - real/electrochemical surface area; CV - cyclic voltammogram; ET - electron transfer; DET - direct electron transfer; k 0 - standard heterogeneous electron transfer rate con- stant; k cat app - apparent bioelectrocatalytic constant; MCO - blue multicopper oxidase; MvBOx - Myrothecium verrucaria bilirubin oxidase; AuNP n - gold nanoparticles with diameter of n nm; AuNP n /Au - gold electrode modified with AuNP n before and after cycling in sulfuric acid (m-AuNP/Au u-AuNP/Au, respectively); ThLc - Trametes hirsuta laccase; PBS - phosphate buffered saline; NHE - normal hydrogen electrode; SEM - scanning electron microscopy; A spr - absorbance maximum; А 450 - absorbance at the wavelength of 450 nm; Γ - enzyme surface concentration; j max - maximum current density. |
| Databáze: | OpenAIRE |
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