Throughput-Speed Product Augmentation for Scanning Fiber-Optic Two-Photon Endomicroscopy
Autor: | Wenxuan Liang, Honghua Guan, Hyeon Cheol Park, Defu Chen, Dwight E. Bergles, Hui Lu, Kaiyan Li, Ang Li, Ming-Jun Li, Yung-Tian A. Gau, Xingde Li, Yuanlei Yue |
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Rok vydání: | 2020 |
Předmět: |
Optical fiber
Materials science Article 030218 nuclear medicine & medical imaging law.invention Mice 03 medical and health sciences 0302 clinical medicine Optics Two-photon excitation microscopy law Animals Fiber Optic Technology Electrical and Electronic Engineering Throughput (business) Photons Radiological and Ultrasound Technology business.industry Frame rate Computer Science Applications Numerical aperture Biophotonics Temporal resolution business Software Preclinical imaging |
Zdroj: | IEEE Trans Med Imaging |
ISSN: | 1558-254X 0278-0062 |
Popis: | Compactness, among several others, is one unique and very attractive feature of a scanning fiber-optic two-photon endomicroscope. To increase the scanning area and the total number of resolvable pixels (i.e., the imaging throughput), it typically requires a longer cantilever which, however, leads to a much undesired, reduced scanning speed (and thus imaging frame rate). Herein we introduce a new design strategy for a fiber-optic scanning endomicroscope, where the overall numerical aperture (NA) or beam focusing power is distributed over two stages: 1) a mode-field focuser engineered at the tip of a double-clad fiber (DCF) cantilever to pre-amplify the single-mode core NA, and 2) a micro objective of a lower magnification (i.e., $\sim 2\times $ in this design) to achieve final tight beam focusing. This new design enables either an ~9-fold increase in imaging area (throughput) or an ~3-fold improvement in imaging frame rate when compared to traditional fiber-optic endomicroscope designs. The performance of an as-designed endomicroscope of an enhanced throughput-speed product was demonstrated by two representative applications: (1) high-resolution imaging of an internal organ (i.e., mouse kidney) in vivo over a large field of view without using any fluorescent contrast agents, and (2) real-time neural imaging by visualizing dendritic calcium dynamics in vivo with sub-second temporal resolution in GCaMP6m-expressing mouse brain. This cascaded NA amplification strategy is universal and can be readily adapted to other types of fiber-optic scanners in compact linear or nonlinear endomicroscopes. |
Databáze: | OpenAIRE |
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