The Major Route for Chlorophyll Synthesis Includes [3,8-divinyl]-chlorophyllide a Reduction in Arabidopsis thaliana
| Autor: | Nozomi Nagata, Ayumi Tanaka, Ryouichi Tanaka |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Chlorophyll
Physiology Arabidopsis Plant Science Reductase Biology Substrate Specificity chemistry.chemical_compound Biosynthesis Arabidopsis thaliana chemistry.chemical_classification Chlorophyllides Temperature Substrate (chemistry) Cell Biology General Medicine Hydrogen-Ion Concentration biology.organism_classification In vitro Enzyme Biochemistry chemistry Etiolation Chlorophyll synthesis Oxidoreductases NADP |
| Zdroj: | Plant and Cell Physiology. 48:1803-1808 |
| ISSN: | 1471-9053 0032-0781 |
| DOI: | 10.1093/pcp/pcm153 |
| Popis: | In most reviews on Chl biosynthesis, Chl is described as being synthesized via the route involving the reduction of [3,8-divinyl]-protochlorophyllide a. However, the possibility remains that the conversion of the divinyl form of the Chl intermediate to its monovinyl form takes place at other enzymatic steps. To determine the actual route of Chl biosynthesis, we examined the substrate specificity of the formerly named [3,8-divinyl]-protochlorophyllide a 8-vinyl reductase (DVR) in vitro. In addition, we investigated the accumulation of various Chl intermediates in etiolated seedlings in vivo. Collectively, these studies indicate that [3,8-divinyl]-chlorophyllide a is the major substrate of DVR. |
| Databáze: | OpenAIRE |
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