Molecular characterization of Giardia duodenalis in Yemen
| Autor: | Naela A. Alyousefi, Yvonne A. L. Lim, Rohela Mahmud, Mohammed A. K. Mahdy, Lihua Xiao |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Giardiasis Male Yemen Adolescent Genotype Genotyping Techniques Sequence analysis Molecular Sequence Data Immunology Protozoan Proteins Microbiology law.invention Feces Young Adult law Animals Humans Child Genotyping Phylogeny Aged Aged 80 and over Base Sequence biology Infant Giardia General Medicine DNA Protozoan Middle Aged 16S ribosomal RNA biology.organism_classification Virology Subtyping Cytoskeletal Proteins Infectious Diseases Transmission (mechanics) Socioeconomic Factors Giardia duodenalis Child Preschool Female Parasitology Giardia lamblia Sequence Alignment |
| Zdroj: | Experimental Parasitology. 134:141-147 |
| ISSN: | 0014-4894 |
| Popis: | Giardia duodenalis is an important intestinal protozoan in Yemen with infection rates ranging from 18% to 27%. To date, there has been no genotyping study to provide a better understanding of the transmission dynamic. This study was conducted to genotype and subtype G. duodenalis in Yemen. Stool samples were collected from 503 Yemeni outpatients between 1 and 80 years old, including 219 males and 284 females. Giardia cysts were detected via microscopy after the formal-ether concentration. Genotyping of Giardia was carried out using PCR and sequence analysis of the 16s rRNA and b-giardin genes. Of the 89 microscopy-positive Giardia samples, 65 were successfully sequenced, of which 66% (43 of 65) were identified as G. duodenalis assemblage A and 34% (22 of 65) as assemblage B. Further subtyping analysis based on b-giardin gene identified the presence of subtypes A2 and A3, which belong to the anthroponotic sub-assemblage AII. Data of the study suggest that anthroponotic transmission played a potential role in the transmission of giardiasis in the community. However, further genotyping and subtyping studies of specimens from humans and animals living in the same households are needed for a more definitive understanding of giardiasis transmission in Yemen. |
| Databáze: | OpenAIRE |
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