Qualification of Human Liver Microsomes for Antibacterial Activity Screening of Drug Metabolites

Autor:	Navid Jubaer
Rok vydání:	2023
Předmět:	Stenotrophomonas maltophilia bacterial contamination MALDI-TOF/TOF microbial ID antibacterial drug discovery clinical mass spectrometry General Earth and Planetary Sciences MRSA drug metabolism Chryseobacterium indologenes General Environmental Science
Zdroj:	Applied Microbiology Volume 3 Issue 1 Pages: 104-118
ISSN:	2673-8007
DOI:	10.3390/applmicrobiol3010009
Popis:	Microsomes are commonly used to perform in vitro drug metabolism, predominantly to form phase I drug metabolites. Pooled microsomes from multiple donors can contain microorganisms from underlying microbial diseases. Exposure to microbes can also occur during extraction if aseptic processing is compromised. Although microbial presence does not affect the metabolic activity of microsomes, presence of unwanted microorganisms can cause interference if the downstream application of microsomal drug metabolites is screening for antibacterial activity. In this work, traditional biochemical tests and advanced proteomics-based identification techniques were used to identify two gram-negative bacteria in pooled human liver microsomes. Several decontamination procedures were assessed to eradicate these two bacteria from the microsomes without affecting its metabolic capacity, and organic extraction was found to be the most convenient and efficient approach to decontaminate microsomes and screen drug metabolites for antibacterial activity against methicillin-resistant Staphylococcus aureus (MRSA).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce2ea705ebf881b1227a7533e8a17d60 https://doi.org/10.3390/applmicrobiol3010009 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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