Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures
| Autor: | Rosanna Pinto, Mariateresa Mancuso, Carmela Marino, Emanuela Pasquali, Claudia Consales, Caterina Merla, Maria Pierdomenico, Vanni Lopresto, Barbara Benassi, Alessio Butera |
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| Přispěvatelé: | Consales, C., Butera, A., Merla, C., Pasquali, E., Lopresto, V., Pinto, R., Pierdomenico, M., Mancuso, M., Marino, C., Benassi, B. |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
3D culture SH-SY5Y Extremely low-frequency magnetic field Neuroscience (miscellaneous) Cell Culture Techniques Neovascularization Physiologic Apoptosis 03 medical and health sciences Cellular and Molecular Neuroscience Neuroblastoma 0302 clinical medicine Superoxide Dismutase-1 In vitro Cell Line Tumor Gene expression medicine Humans RNA Messenger Cell Proliferation biology Chemistry Dopaminergic Neurons Cell Differentiation Cell cycle Nestin medicine.disease Glutathione Cell biology MicroRNAs 030104 developmental biology Magnetic Fields Phenotype Neurology 030220 oncology & carcinogenesis biology.protein Original Article Platelet-derived growth factor receptor Biomarkers |
| Zdroj: | Molecular Neurobiology |
| ISSN: | 1559-1182 0893-7648 |
| Popis: | We here characterize the response to the extremely low-frequency (ELF) magnetic field (MF, 50 Hz, 1 mT) of SH-SY5Y human neuroblastoma cells, cultured in a three-dimensional (3D) Alvetex® scaffold compared to conventional two-dimensional (2D) monolayers. We proved that the growing phenotype of proliferating SH-SY5Y cells is not affected by the culturing conditions, as morphology, cell cycle distribution, proliferation/differentiation gene expression of 3D-cultures overlap what reported in 2D plates. In response to 72-h exposure to 50-Hz MF, we demonstrated that no proliferation change and apoptosis activation occur in both 2D and 3D cultures. Consistently, no modulation of Ki67, MYCN, CCDN1, and Nestin, of invasiveness and neo-angiogenesis-controlling genes (HIF-1α, VEGF, and PDGF) and of microRNA epigenetic signature (miR-21-5p, miR-222-3p and miR-133b) is driven by ELF exposure. Conversely, intracellular glutathione content and SOD1 expression are exclusively impaired in 3D-culture cells in response to the MF, whereas no change of such redox modulators is observed in SH-SY5Y cells if grown on 2D monolayers. Moreover, ELF-MF synergizes with the differentiating agents to stimulate neuroblastoma differentiation into a dopaminergic (DA) phenotype in the 3D-scaffold culture only, as growth arrest and induction of p21, TH, DAT, and GAP43 are reported in ELF-exposed SH-SY5Y cells exclusively if grown on 3D scaffolds. As overall, our findings prove that 3D culture is a more reliable experimental model for studying SH-SY5Y response to ELF-MF if compared to 2D conventional monolayer, and put the bases for promoting 3D systems in future studies addressing the interaction between electromagnetic fields and biological systems. |
| Databáze: | OpenAIRE |
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