抗体結合磁性ナノ粒子による癌細胞特異的温熱療法
| Autor: | Yuki Matsumi, Tetsuya Kagawa, Hiromichi Aono, Toshiyoshi Fujiwara, Toshiaki Ohara, Sho Takeda, Hiroyuki Kishimoto, Yasuhiro Komatsu, Kunitoshi Shigeyasu, Shuya Yano, Hiroshi Tazawa, Robert M. Hoffman |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Hyperthermia Receptor ErbB-2 Drug Compounding Magnetic Field Therapy Apoptosis Biology Proof of Concept Study 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Antineoplastic Agents Immunological Neoplasms medicine Humans Propidium iodide Fluorescein isothiocyanate Molecular Biology Cell Proliferation Drug Carriers Cancer Cell Biology Hyperthermia Induced medicine.disease equipment and supplies HCT116 Cells Coculture Techniques Kinetics 030104 developmental biology Magnetic hyperthermia Magnetic Fields chemistry 030220 oncology & carcinogenesis Cancer cell Cancer research Magnetic nanoparticles Magnetic Iron Oxide Nanoparticles Immunotherapy Fetal bovine serum Developmental Biology Research Paper |
| Zdroj: | Cell Cycle |
| Popis: | Hyperthermia has been used for cancer therapy for a long period of time, but has shown limited clinical efficacy. Induction-heating hyperthermia using the combination of magnetic nanoparticles (MNPs) and an alternating magnetic field (AMF), termed magnetic hyperthermia (MHT), has previously shown efficacy in an orthotopic mouse model of disseminated gastric cancer. In the present study, superparamagnetic iron oxide nanoparticles (SPIONs), a type of MNP, were conjugated with an anti-HER2 antibody, trastuzumab and termed anti-HER2-antibody-linked SPION nanoparticles (anti-HER2 SPIONs). Anti-HER2 SPIONs selectively targeted HER2-expressing cancer cells co-cultured along with normal fibroblasts and HER2-negative cancer cells and caused apoptosis only in the HER2-expressing individual cancer cells. The results of the present study show proof-of-concept of a novel hyperthermia technology, immuno-MHT for selective cancer therapy, that targets individual cancer cells. Abbreviations: AMF: alternating magnetic field; DDW: double distilled water; DMEM: Dulbecco’s Modified Eagle’s; Medium; f: frequency; FBS: fetal bovine serum; FITC: Fluorescein isothiocyanate; GFP: green fluorescent protein; H: amplitude; Hsp: heat shock protein; MHT: magnetic hyperthermia; MNPs: magnetic nanoparticles; PI: propidium iodide; RFP: red fluorescent protein; SPION: superparamagnetic iron oxide (Fe(3)O(4)) nanoparticle. |
| Databáze: | OpenAIRE |
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