Developments of Rice Cell Suspension Culture and A Novel Strategy for Screening New Resistant Lines to Rice Blight Disease Caused by Xanthomonas oryzae pv. oryzae
| Autor: | Chiu-Chsiung Cheng, Restu Rizkyta Kusuma, Liliek Sulistyowati, Yi-Hsien Lin |
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| Rok vydání: | 2018 |
| Předmět: |
Sucrose
Cell lcsh:Plant culture Plant disease resistance Oryza Microbiology lcsh:Agriculture chemistry.chemical_compound Xanthomonas Xanthomonas oryzae pv. oryzae medicine lcsh:SB1-1110 Bacterial blight biology lcsh:S food and beverages biology.organism_classification Resistant cell lines medicine.anatomical_structure chemistry Cell culture Callus Screening Reactive oxygen species Agronomy and Crop Science |
| Zdroj: | AGRIVITA Journal of Agricultural Science, Vol 40, Iss 3, Pp 434-441 (2018) |
| ISSN: | 2302-6766 0126-0537 |
| DOI: | 10.17503/agrivita.v40i3.1779 |
| Popis: | The research aimed to develop a rice cell culture system with high proliferation and screening resistant cell lines of rice to bacterial blight disease caused by Xanthomonas oryza pv. oryzae (Xoo). The culture cells obtained from the callus, cultured on CS-1 medium containing 3 % sucrose and 2 mg L-1 2,4-D for 4 weeks. The results showed that proliferation cell was signifcantly increased 1-fold in 3 weeks of primary culture in CS-1 conditioned medium (fresh/spent medium ratio 1:1) containing 3 % sucrose, 0.5 % glucose, 0.05 % fructose and 2 mg L-1 2,4-D. This medium was used to screen the cell lines through applying culture filtrate of Xoo. The method was to find a novel cell line which could produce high amounts of reactive oxygen species (ROS). Screening results showed 33 % cell lines were strong ROS-producing, two cell lines were selected and cultured for second round screening. The ratio of strong ROS-producing cell lines was increased up to 67 % in the third round screening. The strong ROS-producing cell lines in third round screening can be further cultured for plant regeneration. The rice cell lines with high ROS production may have potential of resistant cell lines against Xoo. |
| Databáze: | OpenAIRE |
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