Reduced neuronal sensitivity and susceptibility of the fall armyworm, Spodoptera frugiperda, to pyrethroids in the absence of known knockdown mutations
| Autor: | Sarah E. McComic, Leslie C. Rault, Daniel R. Swale, Troy D. Anderson |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Insecticides Health Toxicology and Mutagenesis Population Spodoptera medicine.disease_cause 01 natural sciences Insecticide Resistance 03 medical and health sciences chemistry.chemical_compound Pyrethrins medicine Bioassay Animals education Genetics Mutation education.field_of_study biology Point mutation General Medicine biology.organism_classification Phenotype 010602 entomology 030104 developmental biology chemistry Chlorpyrifos Toxicity Fall armyworm Agronomy and Crop Science |
| Zdroj: | Pesticide biochemistry and physiology. 169 |
| ISSN: | 1095-9939 |
| Popis: | Neurophysiological recordings were employed to quantify neuronal sensitivity to neurotoxic insecticides and assessed toxicity across field and laboratory fall armyworm (FAW) populations. Topical toxicity resistance ratios (RR) in field-collected FAW was 767-fold compared to laboratory strains and, importantly, a 1750-fold reduction in potency was observed for λ-cyhalothrin in neurophysiological assays. Field collected FAW were found to have a RR of 12 to chlorpyrifos when compared to the susceptible strain and was 8-fold less sensitive in neurophysiological assays. Surprisingly, there were no point mutations identified in the voltage-gated sodium channel known to cause pyrethroid resistance. For acetylcholinesterase, FAW had more than 80% of their nucleotide sequences consistent with A201 and F290 of the susceptible strains although 60% of the tested population was heterozygous for the G227A mutation. These data indicate that point mutations did not contribute to the high level of pyrethroid resistance and nerve insensitivity in this population of field collected FAW. Additionally, these data suggest the kdr phenotype only explains a portion of the heritable variation in FAW resistance and indicates kdr is not the only predictor of high pyrethroid resistance. Phenotypic assays, such as toxicity bioassays or neurophysiological recordings, using field-collected populations are necessary to reliably predict resistant phenotypes and product failures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect