Flowering Plant Sperm Contains a Cytosolic Soluble Protein Factor Which Can Trigger Calcium Oscillations in Mouse Eggs
| Autor: | Xiu-Ying Huang, Shi-Tao Li, Fang-Zhen Sun |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
endocrine system Microinjections Biophysics Brassica chemistry.chemical_element Calcium Sperm protein Biochemistry Mice Cytosol Human fertilization Equivalent Botany Animals Calcium Signaling Molecular Biology reproductive and urinary physiology Ovum Plant Proteins Mice Inbred ICR biology urogenital system fungi food and beverages Cell Biology biology.organism_classification Spermatozoa Sperm Cell biology chemistry Fertilization Female Flowering plant |
| Zdroj: | Biochemical and Biophysical Research Communications. 287:56-59 |
| ISSN: | 0006-291X |
| DOI: | 10.1006/bbrc.2001.5538 |
| Popis: | There is evidence showing that the sperm-induced Ca(2+) oscillations in mammalian eggs at fertilization are triggered by a sperm-derived protein factor. It was established recently that the activity of the putative sperm protein in causing Ca(2+) oscillations in mammalian eggs is not species-specific in vertebrates (1, 16). Here we report that cytosolic soluble extracts derived from flowering plant sperms in Brassica campestris can also induce fertilization-like Ca(2+) oscillations when microinjected into mouse eggs. The factor responsible for inducing Ca(2+) oscillations in the plant sperm was sperm-specific and heat- or trypsin-labile. Eight to ten sperm equivalents of the plant sperm extracts had enough activity to trigger Ca(2+) oscillations in mouse eggs. Our study suggests that, although plant and mammal are evolutionary divergent species, the activity of the putative sperm protein factor in triggering Ca(2+) signaling in mammalian eggs is not specific to the animal kingdom. |
| Databáze: | OpenAIRE |
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