Development of a loop-mediated isothermal amplification coupled lateral flow dipstick targeting erm(41) for detection of Mycobacterium abscessus and Mycobacterium massiliense

Autor: Yanlin Zhao, Jiale Fan, Qian Chen, Yang Zheng, Yuanyuan Song, Chunfa Liu, Guangxue He, Wencong He, Dongxin Liu, Mingxia Jiang, Xichao Ou, Bing Zhao, Shengfen Wang, Yang Zhou, Hui Xia
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: AMB Express, Vol 9, Iss 1, Pp 1-10 (2019)
AMB Express
ISSN: 2191-0855
DOI: 10.1186/s13568-019-0734-4
Popis: Mycobacterium abscessus (M. abscessus) and Mycobacterium massiliense (M. massiliense) are major pathogens that cause post-surgical wound infection and chronic pulmonary disease. Although they are closely related subspecies of M. abscessus complex, their infections are associated with different drug-resistance and cure rate. In the present study, a loop-mediated isothermal amplification (LAMP) coupled with lateral flow dipstick (LFD) method was developed to simultaneous detect M. abscessus and M. massiliense, via specific erm(41) gene. The amplification was carried out at 65 °C for only 60 min, and the results could be visualized on a lateral flow strip. Positive results only occurred in M. abscessus and M. massiliense, no cross-reaction with other mycobacterial species was observed. Therefore, the cost-effective MABC (M. abscessus complex)–LAMP–LFD method developed here was able to correct the diagnose of M. abscessus and M. massiliense infection in a short time. Thus, this method could be used to guide clinicians in treatment of M. abscessus group infections. Electronic supplementary material The online version of this article (10.1186/s13568-019-0734-4) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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