Domestic swine in a visceral leishmaniasis endemic area produce antibodies against multiple Leishmania infantum antigens but apparently resist to L. infantum infection
| Autor: | Adriano Costa de Alcantara, Eliana A. G. Reis, Valderez Lemos-de-Sousa, Carlos Roberto Franke, Evandro Moraes-Silva, Mitermayer G. Reis, Fred da Silva Julião, Maria Nakatani, Artur G. Dias-Lima, Lain Pontes-de-Carvalho, Márcio Silva Rodrigues, Fabiana Rodrigues Antunes, Roberto Badaró |
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| Rok vydání: | 2006 |
| Předmět: |
Swine
animal diseases Veterinary (miscellaneous) Blotting Western Protozoan Proteins Antibodies Protozoan Antigens Protozoan Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Serology law.invention Seroepidemiologic Studies law Cricetinae parasitic diseases medicine Animals Parasite hosting Leishmania infantum Polymerase chain reaction Swine Diseases biology Kinetoplastida Leishmaniasis DNA Protozoan biology.organism_classification medicine.disease Leishmania Virology Infectious Diseases Visceral leishmaniasis Insect Science Leishmaniasis Visceral Female Parasitology Brazil |
| Zdroj: | Acta Tropica. 98:176-182 |
| ISSN: | 0001-706X |
| DOI: | 10.1016/j.actatropica.2006.04.002 |
| Popis: | In order to investigate whether pigs can be infected by Leishmania infantum, a serological and parasitological study was carried out on swine in the Jequi´e municipality, Northeast of Brazil. Anti-Leishmania infantum antibodies were detected in 37 out of 92 swine (40.2%), by two different assays: an anti-L. infantum lysate and an anti-K39 recombinant protein ELISA. An experimental study was also carried out to verify the susceptibility of domestic pigs to L. infantum infection. Three sows inoculated with 108 stationary-phase infective L. infantum promastigotes (26% metacyclic promastigotes) per kilogram of body weight produced anti- Leishmania antibodies until the end of the experiment, 11 months later. No parasites, however, could be visualized through optical microscopy of spleen, liver and bone marrowor by in vitro culture of these organs. Homogenates of these organs were also inoculated in hamsters, without producing infection. No Leishmania DNA was detected by polymerase chain reaction (PCR) in sand flies fed on these animals. The results indicate that domestic pigs bitten by L. infantum-infected vectors in the endemic area do not display a full infection pattern, and the positive association in endemic areas between the presence of swine and infection in canines may not be ascribable to the former acting as a parasite reservoir |
| Databáze: | OpenAIRE |
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