Identification and Confirmation of Increased Fibrinopeptide A Serum Protein Levels in Gastric Cancer Sera by Magnet Bead Assisted MALDI-TOF Mass Spectrometry
| Autor: | Christoph Röcken, Sören O. Deininger, Juliane Hoffmann, Dagmar Niemeyer, Jörg Sauer, Claudia Knippig, Thomas Wex, Wolfgang Albrecht, Matthias P. A. Ebert, Peter Malfertheiner |
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| Rok vydání: | 2006 |
| Předmět: |
Male
Serum medicine.medical_specialty Peptide fragment Fibrinopeptide A Molecular Sequence Data Serum protein Malignancy Biochemistry Gastroenterology Magnetics Stomach Neoplasms Internal medicine medicine Humans Fibrinopeptide Amino Acid Sequence Aged Chemistry Cancer General Chemistry Middle Aged MALDI-TOF Mass Spectrometry medicine.disease Neoplasm Proteins Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Proteome Immunology Female Biomarkers |
| Zdroj: | Journal of Proteome Research. 5:2152-2158 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr060011c |
| Popis: | Gastric cancer is the second most common malignancy and prognosis remains dismal. The reasons for the poor prognosis are the lack of sensitive serum markers for early detection and screening of high-risk individuals as well as the limited treatment options in advanced cancer stages. Using MALDI-TOF mass spectrometry after prefractionation of sera with magnet hydrophobic C8 coated beads sera from 14 patients with gastric cancer and 14 healthy controls mass spectra were generated. A peptide fragment was found to be highly elevated in cancer sera and was identified as fibrinopeptide A. To confirm proteome analysis of gastric cancer sera, we then screened a larger series of patients with gastric cancer (n = 99), high-risk individuals (n = 13) and normal controls (n = 111) for fibrinopeptide A serum levels. Interestingly, the mean logarithmic concentrations of serum fibrinopeptide A levels were significantly higher in cancer patients (mean 3.636 +/- 0.3738; p0.0001) and high-risk individuals (mean 3.569 +/- 0.4722; p0.05) compared to normal controls (mean 3.303 +/- 0.4012). In contrast, we observed no association of fibrinopeptide A levels with tumor stage, tumor location, presence of regional or distant metastasis, and Lauren type of gastric cancer. In conclusion, MALDI-TOF mass spectrometry of prefractionated gastric cancer sera allows the identification of potential biomarkers that may lead to the development of serum based tests for screening of high-risk individuals. |
| Databáze: | OpenAIRE |
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