The Translational Role of MUC8 in Salivary Glands: A Potential Biomarker for Salivary Stone Disease?

Autor: Martin Schicht, Adrian Reichle, Mirco Schapher, Fabian Garreis, Benedikt Kleinsasser, Malik Aydin, Afsun Sahin, Heinrich Iro, Friedrich Paulsen
Přispěvatelé: Şahin, Afsun (ORCID 0000-0002-5083-5618 & YÖK ID 171267), Schicht, Martin, Reichle, Adrian, Schapher, Mirco, Garreis, Fabian, Kleinsasser, Benedikt, Aydın, Malik, Iro, Heinrich, Paulsen, Friedrich, School of Medicine
Rok vydání: 2021
Předmět:
Zdroj: Diagnostics
Diagnostics; Volume 11; Issue 12; Pages: 2330
Diagnostics, Vol 11, Iss 2330, p 2330 (2021)
ISSN: 2075-4418
Popis: Mucin (MUC) 8 has been shown to play an important role in respiratory disease and inflammatory responses. In the present study, we investigated the question of whether MUC8 is also produced and secreted by salivary glands and whether it may also play a role in the oral cavity in the context of inflammatory processes or in the context of salivary stone formation. Tissue samples from parotid and submandibular glands of body donors (n = 6, age range 63–88 years), as well as surgically removed salivary stones from patients (n = 38, age range 48–72 years) with parotid and submandibular stone disease were immunohistochemically analyzed targeting MUC8 and TNF?. The presence of MUC8 in salivary stones was additionally analyzed by dot blot analyses. Moreover, saliva samples from patients (n = 10, age range 51–72 years), who had a salivary stone of the submandibular gland on one side were compared with saliva samples from the other “healthy” side, which did not have a salivary stone, by ELISA. Positive MUC8 was detectable in the inter-and intralobular excretory ducts of both glands (parotid and submandibular). The glandular acini showed no reactivity. TNF? revealed comparable reactivity to MUC8 in the glandular excretory ducts and also did not react in glandular acini. Salivary stones demonstrated a characteristic distribution pattern of MUC8 that differed between parotid and submandibular salivary stones. The mean MUC8 concentration was 71.06 ng/mL in female and 33.21 ng/mL in male subjects (p = 0.156). Saliva from the side with salivary calculi contained significantly (15-fold) higher MUC8 concentration levels than saliva from the healthy side (p = 0.0005). MUC8 concentration in salivary stones varied from 4.59 ng/mL to 202.83 ng/mL. In females, the MUC8 concentration in salivary stones was significantly (2.3-fold) higher, with an average of 82.84 ng/mL compared to 25.27 ng/mL in male patients (p = 0.034). MUC8 is secreted in the excretory duct system of salivary glands and released into saliva. Importantly, MUC8 salivary concentrations vary greatly between individuals. In addition, the MUC8 concentration is gender-dependent (? > ?). In the context of salivary stone diseases, MUC8 is highly secreted in saliva. The findings support a role for MUC8 in the context of inflammatory events and salivary stone formation. The findings allow conclusions on a gender-dependent component of MUC8.
Deutsche Forschungsgemeinschaft (DFG)
Databáze: OpenAIRE
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