Differential response of antioxidant defense in HepG2 cells on exposure of Livotrit®, in a concentration dependent manner
| Autor: | Sajad Malik, Radhika S. Kusurkar, Saroj S. Ghaskadbi, Smriti P.K. Mittal, Tukaram D. Urmode, Swati Khole |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Antioxidant biology Chemistry medicine.medical_treatment lcsh:R Glutathione reductase 0211 other engineering and technologies lcsh:Medicine 02 engineering and technology 01 natural sciences Molecular biology 0104 chemical sciences Lipid peroxidation 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Enzyme Complementary and alternative medicine Catalase 021105 building & construction Gene expression medicine biology.protein Transcription factor Peroxidase |
| Zdroj: | Journal of Traditional and Complementary Medicine, Vol 9, Iss 1, Pp 38-44 (2019) |
| ISSN: | 2225-4110 |
| DOI: | 10.1016/j.jtcme.2017.08.007 |
| Popis: | Livotrit®, a polyherbal formulation (Zandu, India) is commonly prescribed for liver health. The present study was undertaken to elucidate possible mechanism of antioxidant potential of Livotrit®. Livotrit® exhibited concentration dependent radical scavenging activity, inhibition of lipid peroxidation as well as activation and gene expression of antioxidant enzymes. Interestingly, lower concentration of Livotrit® (0.05%) significantly increased activities and gene expression of catalase, Glutathione reductase (GR) and Gluthathione peroxidase (GPx), while higher concentration of Livotrit® (0.5%) significantly increased antioxidant enzyme Heme-oxygenase 1(HO-1) and not catalase (CAT), GR and GPx. Transcription factor, Nuclear factor erythroid 2-related factor 2 (Nrf2) required for expression of catalase, GR, GPx and HO-1 was efficiently translocated into the nucleus at both concentrations. Inspite of this, concentration dependent activation of these enzymes was found to be mediated through miRNAs involved in regulation of their gene expression. Keywords: Antioxidant enzymes, HO-1, BACH-1, miRNA |
| Databáze: | OpenAIRE |
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