GTPases Arf5 and Arl2 function partially distinctly during oocyte meiosis
Autor: | Yang Ma, Ye‐Xiao Zhang, Fenli Zhang, Dong Zhang, Cong-Rong Li, Yang Wang, Li‐Ya Shi, Chun-Xiang Zhou, Zi-Bin Wang, Na-Na Zhang, Zheng-Rong Xia |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Spindle Apparatus GTPase Biology Microfilament Biochemistry Mice 03 medical and health sciences 0302 clinical medicine Human fertilization Meiosis GTP-Binding Proteins medicine Animals Molecular Biology chemistry.chemical_classification Reactive oxygen species ADP-Ribosylation Factors Autophagy Cell Biology Oocyte Cell biology Actin Cytoskeleton 030104 developmental biology medicine.anatomical_structure chemistry 030220 oncology & carcinogenesis Oocytes Female Function (biology) |
Zdroj: | Journal of Cellular Biochemistry. 122:198-208 |
ISSN: | 1097-4644 0730-2312 |
Popis: | Mammalian female meiosis must be tightly regulated to produce high-quality mature oocytes for subsequent regular fertilization and healthy live birth of the next generation. GTPases control many important signal pathways involved in diverse cellular activities. ADP-ribosylation factor family members (Arfs) in mice possess GTPase activities, and some members have been found to function in meiosis. However, whether other Arfs play a role in meiosis is unknown. In this study, we found that Arl2 and Arf5 are the richest among Arfs in mouse oocytes, and they are more abundant in oocytes than in granular cells. Furthermore, Arl2 and Arf5 depletion both impeded meiotic progression, but by affecting spindles and microfilaments, respectively. Moreover, Arl2 and Arf5 depletion both significantly increased regular reactive oxygen species levels and decreased mitochondrial membrane potential and autophagy, indicating that oocyte quality was damaged by Arl2 and Arf5 depletion. These results suggest that Arl2 and Arf5 are two novel essential GTPases required for oocyte meiosis and quality control. |
Databáze: | OpenAIRE |
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