Thymocyte stimulation by anti-TCR-β, but not by anti-TCR-α, leads to induction of developmental transcription program
| Autor: | Nathalie Niederberger, Jeanette Ampudia, Lukas K. Buehler, Nicholas R. J. Gascoigne |
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| Rok vydání: | 2005 |
| Předmět: |
Transcription
Genetic Receptors Antigen T-Cell alpha-beta T-Lymphocytes T cell Immunology Stimulation Biology Lymphocyte Activation Mice Antibody Specificity Calcium flux Gene expression medicine Animals Immunology and Allergy Receptor Gene Expression Profiling T-cell receptor Antibodies Monoclonal Gene Expression Regulation Developmental Cell Differentiation Cell Biology Molecular biology Endocytosis Mice Inbred C57BL Thymocyte medicine.anatomical_structure Calcium Signal transduction Signal Transduction |
| Zdroj: | Journal of Leukocyte Biology. 77:830-841 |
| ISSN: | 1938-3673 0741-5400 |
| Popis: | Anti-T cell receptor (aTCR) antibody (Ab) stimulation of T cells results in TCR down-modulation and T cell activation. Differences in the effect of anti-α-chain and β-chain Ab have been reported on thymocytes. Anti-β-chain Ab but not anti-α-chain reagents cause long-term TCR down-modulation. However, both types of Ab result in TCR cross-linking and activate early steps in signal transduction. In this study, we show that TCR iternalization and calcium flux, hallmarks of T cell activation, are similar with aVα and aVβ treatment. Therefore, we have compared the gene expression profiles of preselection thymocytes stimulated with these reagents. We find that aVα treatment does not cause any significant change in gene expression compared with control culture conditions. In contrast, aVβ stimulation results in numerous changes in gene expression. The alterations of expression of genes known to be expressed in thymocytes are similar to changes caused by positive thymic selection, suggesting that the expression of some of the genes without known roles in thymocyte development and of novel genes whose expression is found to be altered may also be involved in this process. |
| Databáze: | OpenAIRE |
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