Effects of Thyroid Dysfunction on Reproductive Hormones in Female Rats

Autor: Xusong Hu, Juan Liu, Yanzhou Yang, Dai Heng, Ye Tian, Meng Guo, Xuechun Weng, Yu Ding, Wenbo Liu, Kaili Xu, Cheng Zhang
Rok vydání: 2018
Předmět:
endocrine system
medicine.medical_specialty
Thyroid Hormones
Time Factors
endocrine system diseases
Physiology
Thyroid Gland
030209 endocrinology & metabolism
Ovary
Estrous Cycle
Hyperthyroidism
Gonadotropin-Releasing Hormone
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Hypothyroidism
Physiology (medical)
Internal medicine
medicine
Animals
Testosterone
Cholesterol Side-Chain Cleavage Enzyme
Sexual Maturation
Progesterone
Estrous cycle
030219 obstetrics & reproductive medicine
Estradiol
business.industry
Steroidogenic acute regulatory protein
Cholesterol side-chain cleavage enzyme
Radioimmunoassay
Luteinizing Hormone
Phosphoproteins
Disease Models
Animal
Thyroxine
medicine.anatomical_structure
Endocrinology
Propylthiouracil
Female
Follicle Stimulating Hormone
Follicle-stimulating hormone receptor
business
hormones
hormone substitutes
and hormone antagonists
Biomarkers
Hormone
medicine.drug
Zdroj: The Chinese journal of physiology. 61(3)
ISSN: 0304-4920
Popis: Thyroid hormones (THs) play a critical role in the development of ovarian cells. Although the effects of THs on female reproduction are of great interest, the mechanism remains unclear. We investigated the effects of TH dysregulation on reproductive hormones in rats. Propylthiouracil (PTU) and L-thyroxine were administered to rats to induce hypo- and hyperthyroidism, respectively, and the reproductive hormone profiles were analyzed by radioimmunoassay (RIA). Ovarian histology was evaluated with hematoxylin and eosin (HE) staining, and gene protein level or mRNA content was analyzed by western blotting or reverse transcription polymerase chain reaction (RT-PCR). The serum levels of gonadotropin releasing hormone (GnRH) and follicle stimulating hormone (FSH) in both rat models were significantly decreased on day 21, although there were no significant changes at earlier time points. There were no significant differences in luteinizing hormone (LH) or progesterone (P4) levels between the treatment and the control groups. Both PTU and L-thyroxine treatments downregulated estradiol (E2) concentrations; however, the serum testosterone (T) level was increased only in hypothyroid rats at day 21. In addition, the expression levels of FSH receptor, cholesterol side-chain cleavage enzyme (P450scc), and steroidogenic acute regulatory protein (StAR) were decreased in both rat models. Moreover, the onset of puberty was significantly delayed in the hypothyroid group. These results provide evidence that TH dysregulation alters reproductive hormone profiles, and that the initiation of the estrous cycle is postponed in hypothyroidism.
Databáze: OpenAIRE
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