Ratiometric Fluorescence Detection of Colorectal Cancer-Associated Exosomal miR-92a-3p with DSN-Assisted Signal Amplification by a MWCNTs@Au NCs Nanoplatform
| Autor: | Zhiwei, Sun, Juan, Li, Yao, Tong, Li, Zhao, Xiaoyu, Zhou, Hui, Li, Chuanxin, Wang, Lutao, Du, Yanyan, Jiang |
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| Rok vydání: | 2022 |
| Předmět: |
Nanotubes
Carbon multi-walled carbon nanotubes Au nanoclusters biosensor ratiometric fluorescence duplex-specific nuclease exosomal miRNA Clinical Biochemistry Biomedical Engineering Biosensing Techniques General Medicine Analytical Chemistry MicroRNAs Limit of Detection Humans Colorectal Neoplasms Instrumentation Engineering (miscellaneous) Biotechnology |
| Zdroj: | Biosensors; Volume 12; Issue 7; Pages: 533 |
| ISSN: | 2079-6374 |
| Popis: | The detection of miRNA shows great promise in disease diagnosis. In this work, a ratiometric fluorescent biosensor based on multi-walled carbon nanotubes@gold nanoclusters (MWCNTs@Au NCs) and duplex-specific nuclease (DSN)-assisted signal amplification was fabricated for miRNA detection. Colorectal cancer (CRC)-associated miR-92a-3p extracted from exosomes was selected as the target. MWCNTs@Au NCs performs the dual functions of fluorescence quencher and internal fluorescence reference. In the absence of miR-92a-3p, an Atto-425-modified single-stranded DNA probe is adsorbed on MWCNTs@Au NCs, resulting in the quenching of Atto-425. In the presence of miR-92a-3p, the duplex is formed by hybridization of the probe and miR-92a-3p and leaves the MWCNTs@Au NCs, resulting in the fluorescence recovery of Atto-425. DSN can cleave the probe and result in the release of miR-92a-3p. The released miR-92a-3p can hybridize with other probes to form a signal amplification cycle. The fluorescence of MWCNTs@Au NCs remains stable and constitutes a ratiometric fluorescence system with that of Atto-425. A detection concentration interval of 0.1–10 pM and a limit of detection of 31 fM was obtained under optimized measurement conditions. In addition, the accuracy of the biosensor was validated by detecting the concentration of miR-92a-3p extracted from clinical exosome samples. |
| Databáze: | OpenAIRE |
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