Vascular endothelial cell–specific phosphotyrosine phosphatase (VE-PTP) activity is required for blood vessel development
| Autor: | Astrid Holtmann, Sebastian Baumer, Benjamin August, Dietmar Vestweber, Ruth Funke, Karen Wolburg-Buchholz, Hartwig Wolburg, Alexander Gamp, Urban Deutsch, Linda Keller |
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| Rok vydání: | 2006 |
| Předmět: |
Immunology
Neovascularization Physiologic Apoptosis Protein tyrosine phosphatase Biology Biochemistry Receptor tyrosine kinase Mice Antigens CD medicine Extracellular Animals Amino Acid Sequence Yolk sac Cell Proliferation Sequence Deletion Yolk Sac Receptor-Like Protein Tyrosine Phosphatases Class 3 Endothelial Cells Cell Biology Hematology Cadherins Receptor TIE-2 Mice Mutant Strains Protein Structure Tertiary Cell biology Fibronectin Vascular endothelial growth factor B Endothelial stem cell medicine.anatomical_structure Embryo Loss biology.protein Blood Vessels Protein Tyrosine Phosphatases Blood vessel |
| Zdroj: | Blood. 107:4754-4762 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood-2006-01-0141 |
| Popis: | VE-PTP, a receptor-type phosphotyrosine phosphatase, associates with the tyrosine kinase receptor Tie-2 and VE-cadherin and enhances the adhesive function of the latter. Here, VE-PTP was found to be restricted to endothelial cells, with a preference for arterial endothelium. Mutant mice expressing a truncated, secreted form of VE-PTP lacking the cytoplasmic and transmembrane domains and the most membrane-proximal extracellular fibronectin type III repeat, showed severe vascular malformations causing lethality at 10 days of gestation. Although blood vessels were initially formed, the intraembryonic vascular system soon deteriorated. Blood vessels in the yolk sac developed into dramatically enlarged cavities. In explant cultures of mutant allantoides, endothelial cells were found next to vessel structures growing as cell layers. No signs for enhanced endothelial apoptosis or proliferation were observed. Thus, the activity of VE-PTP is not required for the initial formation of blood vessels, yet it is essential for their maintenance and remodeling. |
| Databáze: | OpenAIRE |
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