Performance of seven serological assays for diagnosing tularemia
| Autor: | Diana Klimpel, Herbert Tomaso, Roland Diller, Peter Otto, George F. Araj, Valerie Chaignat, Marina Djordjevic-Spasic, Anke Ruettger, Konrad Sachse, Wolfgang Müller |
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| Jazyk: | angličtina |
| Předmět: |
Pathology
medicine.medical_specialty Blotting Western Enzyme-Linked Immunosorbent Assay Serology Tularemia 03 medical and health sciences Diagnostic specificity 0302 clinical medicine Medical microbiology Antigen Agglutination Tests Zoonoses medicine Animals Humans 030212 general & internal medicine Francisella tularensis Pathogen Retrospective Studies 0303 health sciences biology 030306 microbiology business.industry biology.organism_classification medicine.disease bacterial infections and mycoses Virology Antibodies Bacterial 3. Good health Infectious Diseases Parasitology Diagnostic sensitivity biology.protein Antibody business Research Article |
| Zdroj: | BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| DOI: | 10.1186/1471-2334-14-234 |
| Popis: | Background Tularemia is a rare zoonotic disease caused by the Gram-negative bacterium Francisella tularensis. Serology is frequently the preferred diagnostic approach, because the pathogen is highly infectious and difficult to cultivate. The aim of this retrospective study was to determine the diagnostic accuracy of tularemia specific tests. Methods The Serazym®Anti-Francisella tularensis ELISA, Serion ELISA classic Francisella tularensis IgG/IgM, an in-house ELISA, the VIRapid® Tularemia immunochromatographic test, an in-house antigen microarray, and a Western Blot (WB) assay were evaluated. The diagnosis tularemia was established using a standard micro-agglutination assay. In total, 135 sera from a series of 110 consecutive tularemia patients were tested. Results The diagnostic sensitivity and diagnostic specificity of the tests were VIRapid (97.0% and 84.0%), Serion IgG (96.3% and 96.8%), Serion IgM (94.8% and 96.8%), Serazym (97.0% and 91.5%), in-house ELISA (95.6% and 76.6%), WB (93.3% and 83.0%), microarray (91.1% and 97.9%). Conclusions The diagnostic value of the commercial assays was proven, because the diagnostic accuracy was >90%. The diagnostic sensitivity of the in-house ELISA and the WB were acceptable, but the diagnostic accuracy was |
| Databáze: | OpenAIRE |
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