Cell Proliferation and DNA Dependent DNA Polymerase Estimation in Acute Lymphoblastic Leukaemia during Treatment with Prednisone and Vincristine
| Autor: | Gunhild Lange Wantzin |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male Vincristine Time Factors Mitotic index Adolescent DNA polymerase Cell DNA-Directed DNA Polymerase chemistry.chemical_compound hemic and lymphatic diseases medicine Humans Lymphocytes Aged biology Cell growth Lymphoblast DNA Neoplasm Hematology Middle Aged Cell cycle Molecular biology Leukemia Lymphoid Leukemia Myeloid Acute medicine.anatomical_structure chemistry Immunology biology.protein Prednisone Female Cell Division DNA medicine.drug |
| Zdroj: | Scandinavian Journal of Haematology. 22:375-385 |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1979.tb00435.x |
| Popis: | The presence of DNA polymerase and primer-template DNA in lymphoblast nuclei by measuring the in vitro incorporation of 3H-thymidine-5′-triphosphate (3H-TTP) was studied in 10 patients with acute lymphoblastic leukaemia. Protein synthesis and various other cytokinetic parameters were also studied. After prednisone (P) administration a marked decrease in 3H-TTP labelling index (3H-TTP LI) was apparent together with an inhibition of 3H-leucine incorporation (3H-LEU LI) into lymphoblasts. A moderate decrease in 3H-TDR labelling index (3H-TDR LI) and a later decrease in mitotic index (MI) were seen. Single cell DNA measurements showed a depletion of 3H-TDR labelled lymphoblasts in early part of S-phase apparent at 24 h lasting up to 54 h after P administration. Vincristine given as a flash injection later in the study period caused an immediate rise of the MI, at the same time the P induced decline in 3H-TTP LI, 3H-TDR LI and 3H-LEU LI were continued in most patients. P is thought to damage the cells both in and outside the cell cycle. In the cell cycle the effect of P is an arresting effect in G1. |
| Databáze: | OpenAIRE |
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