Novel role of Snail 1 in promoting tumor neoangiogenesis
Autor: | Yi-Kun Zhang, Yu-Wei Guo, Yang Yue, Hua Wang |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Inhibitor of Differentiation Protein 1 Vascular Endothelial Growth Factor A Lung Neoplasms Antigens CD34 Fibroblast growth factor Biochemistry 0302 clinical medicine Isothiocyanates RNA Small Interfering tumor derived endothelial cells Lung Research Articles Neoangiogenesis Glucuronidase Tube formation Neovascularization Pathologic Chemistry Endoglin epithelial to mesenchymal transition Gene Expression Regulation Neoplastic Platelet Endothelial Cell Adhesion Molecule-1 Vascular endothelial growth factor A Cell Transformation Neoplastic 030220 oncology & carcinogenesis Sulfoxides Fibroblast Growth Factor 1 Intercellular Signaling Peptides and Proteins Research Article Signal Transduction Epithelial-Mesenchymal Transition Primary Cell Culture Biophysics 03 medical and health sciences von Willebrand Factor Gene silencing Anticarcinogenic Agents Humans Epithelial–mesenchymal transition Molecular Biology Tissue Inhibitor of Metalloproteinase-2 Mesenchymal stem cell Endothelial Cells Membrane Proteins Mesenchymal Stem Cells Cell Biology Hypoxia-Inducible Factor 1 alpha Subunit 030104 developmental biology HIF1A SNAI1 Snai1 Cancer research Snail Family Transcription Factors |
Zdroj: | Bioscience Reports |
ISSN: | 1573-4935 0144-8463 |
Popis: | Snail1 plays an important role in epithelial to mesenchymal transition (EMT) during tumor metastasis; however, whether Snai1 potentiates the process of neoangiogenesis is completely unknown. In the present study, tube formation assay was used to evaluate neoangiogenesis in vitro. The expression of Snai1 and other pro-neoangiogenic factors was measured by quantitative real time PCR. Tumor derived endothelial cells (TDECs) were stimulated with fibroblast growth factor 1 (FGF1) or VEGF and formed more tubes compared with untreated, whereas cells treated with Sulforaphane had less tube formation. Silencing SNAI1 significantly attenuated tube formation accompanied by decreased CD31, CD34, and VWF expression in TDECs compared with control. In contrast, overexpression of Snai1 led to more CD31, CD34, and VWF expression and tube formation. To determine if the observed effects of SNAI1 on tube formation was a global phenomenon, the same assay was conducted in normal mesenchymal stem cells (MSCs). SNAI1 silencing did not have any effect on tube formation in MSCs. The expression of TIMP2, ENG, and HIF1A was up-regulated 3-fold or higher after silencing SNAI1, and ID1, VEGFA, PLG, LECT1, HPSE were shown down-regulated. Taken together, our study elucidates an important role of EMT inducer Snai1 in regulating tumor neoangiogenesis, suggesting a potential therapeutic target for overcoming tumor EMT. |
Databáze: | OpenAIRE |
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