Isolation, Identification and Optimization of a New Extracellular Lipase Producing Strain of Rhizopus sp
| Autor: | Shrikant Pawar, Siddharth A. Mahajan, Jayshree B. Kantak, Asmita Prabhune, Yogesh S. Shouche, Aditi V. Bagade |
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| Rok vydání: | 2011 |
| Předmět: |
DNA
Bacterial Nitrogen Rhizopus oryzae Triacylglycerol lipase Bioengineering Zea mays Applied Microbiology and Biotechnology Biochemistry Corn steep liquor Bacterial Proteins Rhizopus Lipase Molecular Biology Phylogeny Soil Microbiology Zygomycota biology Temperature food and beverages Sequence Analysis DNA General Medicine Hydrogen-Ion Concentration Ribosomal RNA biology.organism_classification Carbon Culture Media Kinetics Glucose Fermentation biology.protein Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 164:969-978 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | A lipolytic mesophilic fungus which produces lipase extracellularly was isolated from soil. Based on ITS1-5.8S-ITS4 region sequences of ribosomal RNA, it was concluded that the isolate JK-1 belongs to genus Rhizopus and clades with Rhizopus oryzae. The present paper reports the screening, isolation, identification, and optimization of fermentation conditions for the production of lipase (EC 3.1.1.3). Culture conditions were optimized, and the highest lipase production was observed in basal medium with corn steep liquor as nitrogen source and glucose as carbon source. Maximum lipase production was observed at 72 h, which is about 870 U/ml. Optimization of fermentation conditions resulted in 16-fold enhancement in enzyme production. |
| Databáze: | OpenAIRE |
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