Comparison of structural integrity and functional status of corneal endothelium stored in Cornisol and Optisol-GS

Autor:	Gowri Priya Chidambaranathan, Ganesh Govardhan Gaikwad, N. Venkatesh Prajna, Yogapriya Sundaresan, Kishan Anilkumar Prajapati
Rok vydání:	2019
Předmět:	Corneal endothelium medicine.medical_specialty Time Factors Phalloidin Confocal Organ Preservation Solutions Cell Count Complex Mixtures Slit Lamp Microscopy functional status Culture Media Serum-Free Cornea chemistry.chemical_compound structural integrity lcsh:Ophthalmology Ophthalmology Humans Medicine Aged Optisol-GS Aged 80 and over Microscopy Confocal Slit lamp Cornisol business.industry Chondroitin Sulfates Endothelium Corneal Dextrans Eye bank Organ Preservation Middle Aged Actin cytoskeleton Actins Tissue Donors eye diseases Staining chemistry lcsh:RE1-994 Zonula Occludens-1 Protein Commentary sense organs Gentamicins Sodium-Potassium-Exchanging ATPase business Immunostaining
Zdroj:	Indian Journal of Ophthalmology, Vol 67, Iss 10, Pp 1579-1584 (2019) Indian Journal of Ophthalmology
ISSN:	0301-4738
DOI:	10.4103/ijo.ijo_2026_18
Popis:	Purpose: To compare the structural integrity and functional status of the donor corneas stored in Cornisol and Optisol-GS. Methods: Fifteen optical grade corneal donor buttons (6 pairs; 3 individual) obtained from Rotary Aravind International Eye Bank were used for the study. The left eye of the paired sample was preserved in Cornisol and the right in Optisol-GS. The three individual buttons were used for the baseline data. The corneas were assessed with slit lamp and specular microscope before and after storage time (7, 10, or 14 days). They were then immunostained for markers of structural integrity (ZO-1, Phalloidin) and functionality (Na+/K+ ATPase). The images were acquired using confocal microscope and analyzed using ImageJ software. Results: There was no difference in the clinical evaluation of the corneal layers between the two media. No marked variation was observed in the immunostaining data with reference to the storage period. Intact cellular integrity was identified in 91% (51%, 98%) [Median (min, max)] of cells in Cornisol and 94% (38%, 98%) cells in Optisol based on ZO-1 staining, comparable to the baseline data [87% (76%, 97%)]. Stress fibers were detected in 42.5% (1%, 88%) cells in Cornisol stored corneas and in 55% (11%, 94%) in Optisol when stained for actin cytoskeleton, which correlated with the presence of epithelial defect before storage and vacuolated endothelial cells after storage. No difference was observed between the two media based on the staining pattern for Na+/K+ ATPase. Conclusion: Cornisol and Optisol-GS are equivalent in maintaining the structural integrity and functionality of the donor corneas.
Databáze:	OpenAIRE
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