Monodisperse Water-in-Oil-in-Water (W/O/W) Double Emulsion Droplets as Uniform Compartments for High-Throughput Analysis via Flow Cytometry

Autor:	Wolfgang-Andreas C. Bauer, Wilhelm T. S. Huck, Martin Fischlechner, Clemens F. Kaminski, Florian Hollfelder, Yan Jing
Jazyk:	angličtina
Rok vydání:	2013
Předmět:	Fluorophore lcsh:Mechanical engineering and machinery Microfluidics Dispersity microfluidics Analytical chemistry 02 engineering and technology Double emulsion 01 natural sciences Flow cytometry chemistry.chemical_compound medicine double emulsion lcsh:TJ1-1570 Electrical and Electronic Engineering Water in oil medicine.diagnostic_test 010405 organic chemistry Chemistry flow cytometry Mechanical Engineering double flow-focusing geometry 021001 nanoscience & nanotechnology Fluorescence 6. Clean water 0104 chemical sciences High throughput analysis Control and Systems Engineering 0210 nano-technology
Zdroj:	Micromachines Micromachines, Vol 4, Iss 4, Pp 402-413 (2013)
DOI:	10.3390/mi4040402
Popis:	Here we report the application of monodisperse double emulsion droplets, produced in a single step within partially hydrophilic/partially hydrophobic microfluidic devices, as defined containers for quantitative flow cytometric analysis. Samples with varying fluorophore concentrations were generated, and a clear correlation between dye concentration and fluorescence signals was observed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce1d016a640b24ad951e798d990a40ac Zobrazit plný text záznamu