Species diversity, mating type assays and aggressiveness patterns of Monilinia pathogens causing brown rot of peach fruit in Turkey

Autor: Hacer Handan Altinok, E. Silan, Hilal Ozkilinc, M. R. Durak, K. Arslan, H. Guven, Gozde Yildiz, R. Altindag
Rok vydání: 2020
Předmět:
Zdroj: European Journal of Plant Pathology. 157:799-814
ISSN: 1573-8469
0929-1873
DOI: 10.1007/s10658-020-02040-7
Popis: Brown rot disease caused by fungal species from theMonilinia genus has recently been observed as one of the most importantlimiting factors for yield and quality of peach fruits in Turkey. During Juneand July of 2018, field trips to different peach orchards were performed in sixprovinces located in four different geographical regions of Turkey. One hundredand twenty-nine isolates were obtained from the diseased fruits, and of those,109 were identified as Monilinia fructicola, whereas 20 were M. laxa accordingto the species-specific molecular markers. Mating types of the isolates weredetected by PCR assays using primers designed in this study. Each isolaterepresented only one of the mating type genes. Overall data sets presented 1:1ratio of mating types for both species, indicating a possible sexualreproduction. No polymorphisms were detected in partial sequences of matingtype genes. The isolates were in vitro tested for their pathogenicity using applefruits as a host and aggressiveness were evaluated at different time pointsusing lesion sizes. Monilinia laxa was found more aggressive than M.fructicola. Moreover, M. fructicola isolates displayed larger colony growthcompared to M. laxa isolates. However, no significant correlation was detectedbetween the colony growth rates and virulence. This study is the first to demonstratebrown rot pathogens of peach fruit in Turkey and identifies different aspectsof the pathogens, which would be useful in containment of pathogen spread andcomparison with other populations of these pathogens worldwide, and suggests anew set of mating type markers for these species.
Databáze: OpenAIRE
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