Adjustable hardness of hydrogel for promoting vascularization and maintaining stemness of stem cells in skin flap regeneration
| Autor: | Guoqing Pan, Wenguo Cui, Yuguang Zhang, Ruoyu Cheng, Xiaoming Sun, Hélder A. Santos, Hongbo Zhang, Liying Cheng, Lu Zhang, Youwen Cao, Jinlin He, Kun-Ming Che, Peihong Ni, Lianfu Deng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Skin flap
Adipose tissue 02 engineering and technology Matrix (biology) 010402 general chemistry 01 natural sciences SCAFFOLDS Skin ischemia DEXTRAN chemistry.chemical_compound Cell injection Bioluminescence imaging General Materials Science Stemness 1182 Biochemistry RELEASE Regeneration (biology) Vascularization technology industry and agriculture STIFFNESS 021001 nanoscience & nanotechnology 0104 chemical sciences Cell biology cell and molecular biology Hydrogel Dextran chemistry 216 Materials engineering 1182 Biochemistry cell and molecular biology Adipose derived stem cells Stem cell 0210 nano-technology |
| Zdroj: | Applied Materials Today, 13, 54-63 |
| ISSN: | 2352-9407 |
| Popis: | The matrix mechanical stiffness of biomaterials plays an important role in the pluripotency and biological function of stem cells in the microenvironment. It is a key step to adjust the stiffness of biomaterials for inducing stem cells to promote vascularization in order to promote damaged tissue repair. In this study, we transplant adipose derived stem cells (ADSCs) within an in situ forming dextran hydrogel with controllable mechanical strength formed by cross-linking glycidyl methacrylate derivatized dextran and dithiothreitol, which can regulate the stemness and biological functions of stem cells. We show that softer dextran hydrogel can better maintain stemness markers expression of ADSCs, and significantly stimulate ADSCs to secrete angiogenic factors. The ADSCs-encapsulated hydrogel distinctly promote the skin flap survival compared to direct cell injection. Bioluminescence imaging analysis shows that in situ forming dextran hydrogel can improve cells retention, and postmortem analysis reveals that the transplanted ADSCs with hydrogel can promote vascularization. These results support the use of injectable dextran hydrogel for skin ischemia tissue regeneration. (C) 2018 Elsevier Ltd. All rights reserved. |
| Databáze: | OpenAIRE |
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