Localization of Legionella pneumophila in Tissue Using FITC-Conjugated Specific Antibody and a Background Stain
| Autor: | Barbara S Lowry, Filiberto G Vega, Kenneth W Hedlund |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Ultraviolet Rays
Legionella Guinea Pigs Legionella pneumophila Stain Microbiology Mice Mice Inbred AKR chemistry.chemical_compound Ultraviolet light Animals Crystal violet Fluorescein Staining and Labeling biology Metachromasia food and beverages General Medicine Fluoresceins biology.organism_classification Antibodies Bacterial Staining Microscopy Fluorescence chemistry Gentian Violet Fluorescein-5-isothiocyanate Thiocyanates |
| Zdroj: | American Journal of Clinical Pathology. 77:601-605 |
| ISSN: | 1943-7722 0002-9173 |
| DOI: | 10.1093/ajcp/77.5.601 |
| Popis: | Lightly staining formalin-fixed or fresh tissue with Gram's crystal violet obviates interfering nonspecific fluorescence by acting as a metachromatic stain in ultraviolet light. Against the easily recognized background of tissues and cells fluorescein isothiocyanate-tagged Legionella pneumophila antibodies can then identify this bacterium in or on individual cells. This procedure can be run at room temperature in two hours and has the potential for further widespread applicability. |
| Databáze: | OpenAIRE |
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