Novel pathway for megakaryocyte production after in vivo conditional eradication of integrin αIIb-expressing cells
| Autor: | Thierry Kortulewski, Pierre Vaigot, Pascal Soularue, Olivier Alibert, Alexandre Pawlik, Diana Tronik-Le Roux, Beatrice Jacquelin, Xavier Gidrol, Christophe Joubert, Gaëtan Gruel |
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| Přispěvatelé: | Laboratoire de Cancérologie Expérimentale (LCE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Service de Génomique Fonctionnelle, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Supported by funds from the Commissariat à l'Energie Atomique (CEA) and the Association Française contre les Myopathies (AFM) and by a postdoctoral training grant from Genopole-Evry., We thank Drs A. Galy and E. Lauret for their assistance in cell culture studies, J.-M. Egly and Y. Senis for critical reading of the manuscript, and Dr G. Marguerie, who initially established the CEA microarray platform. We also thank the staff of the Genethon mouse facility and P. Flament and V. Neuville for outstanding work in animal care at the CEA-FAR mouse facility. All the animal studies reported were performed in accordance with current French regulations (Décret no. 87-848 modifié, Ministère de l'Agriculture). |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Myeloid
Cellular differentiation Biochemistry Mice 0302 clinical medicine Megakaryocyte Myeloid Cells MESH: Animals MESH: Proteins Lymphocytes MESH: Platelet Membrane Glycoprotein IIb* / genetics 0303 health sciences education.field_of_study MESH: Bone Marrow Cells / physiology Polycomb Repressive Complex 2 MESH: Regeneration Hematology 3. Good health Cell biology DNA-Binding Proteins Haematopoiesis medicine.anatomical_structure Myelopoiesis Megakaryocytes MESH: Polycomb Repressive Complex 2 Platelet Membrane Glycoprotein IIb MESH: DNA Nucleotidylexotransferase / genetics MESH: Mice Transgenic Immunology Population Bone Marrow Cells Mice Transgenic MESH: Lymphocytes / physiology Biology MESH: Hematopoiesis 03 medical and health sciences MESH: Gene Expression Profiling DNA Nucleotidylexotransferase [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] MESH: Megakaryocytes / cytology medicine MESH: Enhancer of Zeste Homolog 2 Protein [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Animals Regeneration Cell Lineage Enhancer of Zeste Homolog 2 Protein Lymphopoiesis education MESH: Mice MESH: Myeloid Cells / physiology 030304 developmental biology MESH: DNA-Binding Proteins / genetics Gene Expression Profiling MESH: Histone-Lysine N-Methyltransferase Proteins Histone-Lysine N-Methyltransferase Cell Biology MESH: Cell Lineage Molecular biology Hematopoiesis Gene expression profiling 030215 immunology |
| Zdroj: | Blood Blood, 2005, 106 (6), pp.1965-1974. ⟨10.1182/blood-2004-10-3975⟩ Blood, American Society of Hematology, 2005, 106 (6), pp.1965-1974. ⟨10.1182/blood-2004-10-3975⟩ |
| ISSN: | 0006-4971 1528-0020 |
| DOI: | 10.1182/blood-2004-10-3975⟩ |
| Popis: | Our knowledge of the molecular mechanisms that regulate hematopoiesis in physiologic and pathologic conditions is limited. Using a molecular approach based on cDNA microarrays, we demonstrated the emergence of an alternative pathway for mature bone marrow cell recovery after the programmed and reversible eradication of CD41+ cells in transgenic mice expressing a conditional toxigene targeted by the platelet αIIb promoter. The expression profile of the newly produced CD41+ cells showed high levels of transcripts encoding Ezh2, TdT, Rag2, and various immunoglobulin (Ig) heavy chains. In this context, we identified and characterized a novel population of Lin-Sca-1hic-Kit- cells, with a lymphoid-like expression pattern, potentially involved in the reconstitution process. Our study revealed novel transcriptional cross talk between myeloid and lymphoid lineages and identified gene expression modifications that occur in vivo under these particular stress conditions, opening important prospects for therapeutic applications. |
| Databáze: | OpenAIRE |
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