Absence of Therapeutic Benefit of the Anti-Inflammatory Protein TSG-6 for Corneal Alkali Injury in a Rat Model

Autor:	Samuel F. Fulcher, Roxanne L. Reger, Hosoon Choi, Darwin J. Prockop, Casie Phillips, Joo Youn Oh, Luke B. Potts
Rok vydání:	2019
Předmět:	Male medicine.medical_specialty genetic structures Anterior Chamber medicine.medical_treatment Administration Ophthalmic Inflammation Real-Time Polymerase Chain Reaction Corneal Diseases Neovascularization 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Fibrosis In vivo Ophthalmology Burns Chemical medicine Animals Sodium Hydroxide Infusions Intravenous Saline TSG-6 business.industry medicine.disease eye diseases Sensory Systems Rats Disease Models Animal Eye Burns Rats Inbred Lew Corneal neovascularization 030221 ophthalmology & optometry Tumor necrosis factor alpha sense organs Injections Intraocular medicine.symptom business Cell Adhesion Molecules 030217 neurology & neurosurgery
Zdroj:	Current Eye Research. 44:873-881
ISSN:	1460-2202 0271-3683
DOI:	10.1080/02713683.2019.1597893
Popis:	Purpose: To investigate the therapeutic efficacy of tumor necrosis factor (TNF)-α stimulated gene/protein 6 (TSG-6) in a rat model of corneal alkali injury. Methods: Corneal alkali injury was produced by placing an NaOH-soaked filter paper disk on the central cornea of the right eye of an anesthetized male Lewis (LEW/Crl) rat. Recombinant human TSG-6, or an equal volume of phosphate-buffered saline (PBS), was administered intravenously (IV), by anterior chamber (AC) injection, or as a topical drop. The affected eyes were photographed daily using a dissecting microscope and documented for clinical time course analysis of corneal opacification. Corneal tissue was excised at pre-determined therapeutic endpoints, with subsequent qRT-PCR or histological analyses. Results: The continuous monitoring of corneal alkali injury progression revealed TSG-6 treatments do not show sufficient effectiveness in vivo regardless of IV injection, AC injection, or topical application. Corneal opacification and neovascularization were not diminished, and gene expression was not impacted by these treatments. However, both IV and AC administration of TSG-6 significantly suppressed pro-inflammatory cytokines compared to PBS-treated eyes. Conclusion: We conclude that the therapeutic potential of TSG-6 is insufficient in a rat corneal alkali injury model.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce1b77d00ea7e3dc1385bde3a1c5e9ed https://doi.org/10.1080/02713683.2019.1597893 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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