Restrained expansion of the recall germinal center response as biomarker of protection for influenza vaccination in mice

Autor: Laura Solforosi, Dominika Czapska-Casey, Harmjan Kuipers, Laurens P Kil, Ramon Roozendaal, Joost Vaneman, Jeroen Tolboom, Joan E. M. van der Lubbe, Roland Zahn
Jazyk: angličtina
Rok vydání: 2019
Předmět:
0301 basic medicine
B Cells
Physiology
Antibody Response
Hemagglutinin Glycoproteins
Influenza Virus
Antibodies
Viral
Biochemistry
White Blood Cells
Mice
0302 clinical medicine
Animal Cells
Immune Physiology
Medicine and Health Sciences
Public and Occupational Health
Enzyme-Linked Immunoassays
Immune Response
Vaccines
Multidisciplinary
Immune System Proteins
biology
Vaccination and Immunization
Vaccination
Infectious Diseases
Influenza Vaccines
030220 oncology & carcinogenesis
Medicine
Antibody
Cellular Types
Research Article
Infectious Disease Control
Influenza vaccine
Immune Cells
Science
Immunology
Hemagglutinin (influenza)
Research and Analysis Methods
Virus
Antibodies
03 medical and health sciences
Antigen
Antigen Isotypes
Orthomyxoviridae Infections
Animals
Antigens
Antibody-Producing Cells
Immunoassays
Blood Cells
Germinal center
Biology and Life Sciences
Proteins
Cell Biology
Germinal Center
Antibodies
Neutralizing
Disease Models
Animal
030104 developmental biology
Immunization
biology.protein
Immunologic Techniques
Preventive Medicine
Immunologic Memory
Biomarkers
Zdroj: PLoS ONE, Vol 14, Iss 11, p e0225063 (2019)
PLoS ONE
ISSN: 1932-6203
Popis: Correlates of protection (CoP) are invaluable for iterative vaccine design studies, especially in pursuit of complex vaccines such as a universal influenza vaccine (UFV) where a single antigen is optimized to elicit broad protection against many viral antigenic variants. Since broadly protective antibodies against influenza virus often exhibit mutational evidence of prolonged diversification, we studied germinal center (GC) kinetics in hemagglutinin (HA) immunized mice. Here we report that as early as 4 days after secondary immunization, the expansion of HA-specific GC B cells inversely correlated to protection against influenza virus challenge, induced by the antigen. In contrast, follicular T helper (TFH) cells did not expand differently after boost vaccination, suggestive of a B-cell intrinsic difference in activation and differentiation inferred by protective antigen properties. Importantly, differences in antigen dose only affected GC B-cell frequencies after primary immunization. The absence of accompanying differences in total anti-HA or epitope-specific antibody levels induced by vaccines of different efficacy suggests that the GC B-cell response upon revaccination represents an early and unique marker of protection that may significantly accelerate the pre-clinical phase of vaccine development.
Databáze: OpenAIRE
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