Potyvirus Genome-Linked Protein (VPg) Determines Pea Seed-Borne Mosaic Virus Pathotype-Specific Virulence in Pisum sativum
| Autor: | R. O. Hampton, Robert R. Martin, K. E. Keller, I. E. Johansen |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Genotype
Physiology Molecular Sequence Data Potyvirus Virulence Genome Viral Viral Nonstructural Proteins Virus Coding region Amino Acid Sequence Genetics Infectivity Sequence Homology Amino Acid biology Potyviridae Protoplasts Genetic Complementation Test Peas RNA-Binding Proteins food and beverages RNA General Medicine biology.organism_classification Virology Ribonucleoproteins Pea seed-borne mosaic virus Agronomy and Crop Science |
| Zdroj: | Molecular Plant-Microbe Interactions®. 11:124-130 |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi.1998.11.2.124 |
| Popis: | The mechanism of Pisum sativum pathotype-specific resistance to pea seed-borne mosaic potyvirus (PSbMV) was investigated and the coding region determinant of PSbMV virulence was defined. Homozygous recessive sbm-1 peas are unable to support replication of PSbMV pathotype 1 (P-1), whereas biochemically and serologically related pathotype 4 (P-4) is fully infectious in the sbm-1/sbm-1 genotype. We were unable to detect viral coat protein or RNA with double antibody sandwich-enzyme-linked im-munosorbent assay and reverse transcription-polymerase chain reaction in sbm-1/sbm-1 P-1-inoculated protoplasts and plants. Lack of viral coat protein or RNA in P-1 trans-fected sbm-1/sbm-1 protoplasts suggests that sbm-1 resistance is occurring at the cellular level and that inhibition of cell-to-cell virus movement is not the operating form of resistance. In addition, because virus products were not detected at any time post-inoculation, resistance must either be constitutive or expressed very early in the virus infection process. P-1-resistant peas challenged with full-length, infectious P-1/P-4 recombinant clones demonstrated that a specific P-4 coding region, the 21-kDa, genome-linked protein (VPg), was capable of overcoming sbm-1 resistance, whereas clones containing the P-1 VPg coding region were noninfectious to sbm-1/sbm-1 peas. VPg is believed to be involved in potyvirus replication and its identification as the PSbMV determinant of infectivity in sbm-1/sbm-1 peas is consistent with disruption of an early P-1 replication event. |
| Databáze: | OpenAIRE |
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