Insight into Isoprenoid Biosynthesis by Functional Analysis of Isoprenyl Diphosphate Synthases from Mycobacterium vanbaalenii and Mycobacterium tuberculosis
| Autor: | Tsutomu Sato, Daijiro Ueda, Tohru Abe, Sadamu Ozaki |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
010402 general chemistry
01 natural sciences Biochemistry Mycobacterium tuberculosis chemistry.chemical_compound Biosynthesis Glycosyl Molecular Biology Mycobacteriaceae chemistry.chemical_classification Alkyl and Aryl Transferases Functional analysis biology ATP synthase Molecular Structure 010405 organic chemistry Terpenes Organic Chemistry biology.organism_classification Terpenoid 0104 chemical sciences Enzyme chemistry biology.protein Molecular Medicine Mycobacterium vanbaalenii lipids (amino acids peptides and proteins) |
| Zdroj: | Chembiochem : a European journal of chemical biology. 21(20) |
| ISSN: | 1439-7633 |
| Popis: | Comprehensive functional analyses of E-isoprenyl diphosphate synthases (E-IDSs) from nonpathogenic Mycobacterium vanbaalenii have been performed. Mv0992 and Mv1577 represent a nonaprenyl diphosphate (E-C45 ) synthase and a geranylgeranyl diphosphate (E-C20 ) synthase, respectively. Although Mv3536 was identified as an E-C20 synthase using a single enzyme, co-incubation of Mv3536 and Z-IDSs (Mv4662 and Mv3822) strongly suggested it releases an intermediate geranyl diphosphate (E-C10 ) during a continuous condensation reaction. Mv0992 and Mv3536 functions differed from those of the previously reported pathogenic Mycobacterium tuberculosis homologues Rv0562 and Rv2173, respectively. Re-analysis of Rv0562 and Rv2173 demonstrated that their functions were similar to those of Mv0992 and Mv3536 (Rv0562: E-C45 synthase; Rv2173: E-C10-15 synthase). The newly proposed functions of Rv0562 and Rv2173 would be in the biosynthesis of menaquinone and glycosyl carrier lipids essential for growth. Furthermore, a reduced allylic diphosphate could be used as the Z-IDS of the Mv3822 substrate, thereby introducing a potentially novel pathway of cyclic sesquarterpene biosynthesis. |
| Databáze: | OpenAIRE |
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