OsIRO3 Plays an Essential Role in Iron Deficiency Responses and Regulates Iron Homeostasis in Rice
| Autor: | Jun Ye, Wujian Wang, Huixia Shou, Yanran Ma, Ting Wang, Luqing Zheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Necrosis Mutant Plant Science 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound iron lcsh:Botany medicine Nicotianamine Iron deficiency (plant disorder) Transcription factor Gene Ecology Evolution Behavior and Systematics transcription factor OsNAS3 Ecology Chemistry rice OsIRO3 food and beverages nicotianamine lcsh:QK1-989 Cell biology 030104 developmental biology Shoot medicine.symptom Fe deficiency Homeostasis 010606 plant biology & botany |
| Zdroj: | Plants Volume 9 Issue 9 Plants, Vol 9, Iss 1095, p 1095 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9091095 |
| Popis: | Iron (Fe) homeostasis is essential for plant growth and development, and it is strictly regulated by a group of transcriptional factors. Iron-related transcription factor 3 (OsIRO3) was previously identified as a negative regulator for Fe deficiency response in rice. However, the molecular mechanisms by which OsIRO3 regulate Fe homeostasis is unclear. Here, we report that OsIRO3 is essential for responding to Fe deficiency and maintaining Fe homeostasis in rice. OsIRO3 is expressed in the roots, leaves, and base nodes, with a higher level in leaf blades at the vegetative growth stage. Knockout of OsIRO3 resulted in a hypersensitivity to Fe deficiency, with severe necrosis on young leaves and defective root development. The iro3 mutants accumulated higher levels of Fe in the shoot under Fe-deficient conditions, associated with upregulating the expression of OsNAS3, which lead to increased accumulation of nicotianamine (NA) in the roots. Further analysis indicated that OsIRO3 can directly bind to the E-box in the promoter of OsNAS3. Moreover, the expression of typical Fe-related genes was significantly up-regulated in iro3 mutants under Fe-sufficient conditions. Thus, we conclude that OsIRO3 plays a key role in responding to Fe deficiency and regulates NA levels by directly, negatively regulating the OsNAS3 expression. |
| Databáze: | OpenAIRE |
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