Assessment of flow within developing chicken vasculature and biofabricated vascularized tissues using multimodal imaging techniques
Autor: | Tom Knop, Prasanna Padmanaban, Wiendelt Steenbergen, Vasileios D. Trikalitis, Bart F.J.M. Koopman, Ata Chizari, Jiena Zhang, Jeroen Rouwkema |
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Přispěvatelé: | Biomechanical Engineering, TechMed Centre, Biomedical Photonic Imaging |
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Erythrocytes
UT-Gold-D Computer science Science Neovascularization Physiologic Chick Embryo Contrast imaging Imaging data Multimodal Imaging Article Imaging 03 medical and health sciences 0302 clinical medicine Medical research Lasers LEDs and light sources Animals Optical techniques 030304 developmental biology Biological models Multimodal imaging 0303 health sciences Microscopy Multidisciplinary Tissue Engineering Muscles Optical Imaging Blood flow Flow perfusion Flow (mathematics) Blood Circulation Blood Vessels Medicine Applied optics 030217 neurology & neurosurgery Biomedical engineering |
Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Scientific reports, 11:18251, 1-14. Nature Publishing Group Scientific Reports |
ISSN: | 2045-2322 |
Popis: | Fluid flow shear stresses are strong regulators for directing the organization of vascular networks. Knowledge of structural and flow dynamics information within complex vasculature is essential for tuning the vascular organization within engineered tissues, by manipulating flows. However, reported investigations of vascular organization and their associated flow dynamics within complex vasculature over time are limited, due to limitations in the available physiological pre-clinical models, and the optical inaccessibility and aseptic nature of these models. Here, we developed laser speckle contrast imaging (LSCI) and side-stream dark field microscopy (SDF) systems to map the vascular organization, spatio-temporal blood flow fluctuations as well as erythrocytes movements within individual blood vessels of developing chick embryo, cultured within an artificial eggshell system. By combining imaging data and computational simulations, we estimated fluid flow shear stresses within multiscale vasculature of varying complexity. Furthermore, we demonstrated the LSCI compatibility with bioengineered perfusable muscle tissue constructs, fabricated via molding techniques. The presented application of LSCI and SDF on perfusable tissues enables us to study the flow perfusion effects in a non-invasive fashion. The gained knowledge can help to use fluid perfusion in order to tune and control multiscale vascular organization within engineered tissues. |
Databáze: | OpenAIRE |
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