Nanotopography-Promoted Formation of Axon Collateral Branches of Hippocampal Neurons
| Autor: | Sunghoon Joo, Kyungtae Kang, Insung S. Choi, Jeongyeon Seo, Ji Yu Choi, Woo Kyung Cho, Juan Kim |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
02 engineering and technology General Chemistry Biology Hippocampal formation 021001 nanoscience & nanotechnology Biomaterials 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure nervous system medicine General Materials Science Nanotopography Neuron 0210 nano-technology Structural motif Growth cone Cytoskeleton Filopodia Neuroscience Immunostaining Biotechnology |
| Zdroj: | Small (Weinheim an der Bergstrasse, Germany). |
| ISSN: | 1613-6829 |
| Popis: | Axon collateral branches, as a key structural motif of neurons, allow neurons to integrate information from highly interconnected, divergent networks by establishing terminal boutons. Although physical cues are generally known to have a comprehensive range of effects on neuronal development, their involvement in axonal branching remains elusive. Herein, it is demonstrated that the nanopillar arrays significantly increase the number of axon collateral branches and also promote their growth. Immunostaining and biochemical analyses indicate that the physical interactions between the nanopillars and the neurons give rise to lateral filopodia at the axon shaft via cytoskeletal changes, leading to the formation of axonal branches. This report, demonstrates that nanotopography regulates axonal branching, and provides a guideline for the design of sophisticated neuron-based devices and scaffolds for neuro-engineering. |
| Databáze: | OpenAIRE |
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