Fast reversibly photoswitching red fluorescent proteins for live-cell RESOLFT nanoscopy
| Autor: | Alexander S. Mishin, Andreas Bodén, Ilaria Testa, Francesca Pennacchietti, Vladislav V. Verkhusha, Nadya G. Gurskaya, Irina I. Shemyakina, Ekaterina O. Serebrovskaya, Jes Dreier, Dmitry M. Chudakov, Aline R. Faro, A A Kotlobay, Konstantin A. Lukyanov, Nina G. Bozhanova |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Intravital Microscopy Light Chemistry Super-resolution microscopy Recombinant Fusion Proteins RESOLFT Cell Biology Photochemical Processes Biochemistry Fluorescence Cell Line 03 medical and health sciences Kinetics Luminescent Proteins 030104 developmental biology 0302 clinical medicine Microscopy Fluorescence Spectrophotometry Biophysics Humans Nanotechnology Molecular Biology 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | Nature Methods |
| ISSN: | 1548-7105 |
| Popis: | Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells. |
| Databáze: | OpenAIRE |
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