Phyllanthus amarus down-regulates hepatitis B virus mRNA transcription and replication
| Autor: | M. Ott, S. Gupta, C.-D. Lee, D. A. Shafritz, Robert D. Burk, Sadras Panchatcharam Thyagarajan |
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| Rok vydání: | 1996 |
| Předmět: |
DNA Replication
Hepatitis B virus HBsAg Transcription Genetic Cell Survival Hepatitis B virus DNA polymerase viruses Clinical Biochemistry Down-Regulation Mice Transgenic Viral transformation Virus Replication medicine.disease_cause Antiviral Agents Biochemistry Virus Hepatitis B virus PRE beta Mice Tumor Cells Cultured medicine Animals RNA Messenger Nucleic Acid Synthesis Inhibitors Plants Medicinal biology Plant Extracts General Medicine biology.organism_classification Virology HBcAg Hepadnaviridae |
| Zdroj: | European Journal of Clinical Investigation. 26:1069-1076 |
| ISSN: | 1365-2362 0014-2972 |
| Popis: | The Phyllanthus amarus plant shows potential for treating hepatitis B virus. To define the mechanism of action of P. amarus, we used HepG2 2.2.15 cells, which support hepatitis B virus replication. P. amarus inhibited hepatitis B virus polymerase activity, decreased episomal hepatitis B virus DNA content and suppressed virus release into culture medium. To examine transcriptional control mechanisms, we used G26 hepatitis B virus transgenic mice, which produce serum HBsAg but neither HBcAg nor virion particles. When P. amarus was administered to transgenic mice, hepatic HBsAg mRNA levels decreased, indicating transcriptional or post-transcriptional down-regulation of the transgene. Increase in hepatitis B virus mRNA expression after stimulation of the glucocorticoid responsive element was also suppressed by P. amarus, suggesting involvement of the hepatitis B virus enhancer in this response. Disruption by P. amarus of hepatitis B virus polymerase activity, mRNA transcription and replication supports its role as an antiviral agent. |
| Databáze: | OpenAIRE |
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